Anti-beta Synuclein 抗体 (ab6165)

Reviews (1)Q&A (2)References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)

    Key features and details

    • Rabbit polyclonal to beta Synuclein
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-beta Synuclein antibody
      beta Synuclein 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to beta Synuclein

    • 由来種

      Rabbit

    • 特異性

      Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6165の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (1)
    1/500 - 1/2000.
    特記事項
    IHC-P
    1/500 - 1/2000.

    ターゲット情報

    • 機能

      Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

    • 組織特異性

      Expressed predominantly in brain; concentrated in presynaptic nerve terminals.

    • 配列類似性

      Belongs to the synuclein family.

    • 翻訳後修飾

      Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 14 kDa brain-specific protein antibody
        • Beta-synuclein antibody
        • Phosphoneuroprotein 14 antibody
        • PNP14 antibody
        • Sncb antibody
        • Synuclein beta antibody
        • SYUB_HUMAN antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
        ab6165 staining beta synuclein in human occipital cortex.
        Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
        Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      参考文献 (4)

      ab6165 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6165 は 4 報の論文で使用されています。

      • Lahut S  et al. Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease. Dis Model Mech 10:619-631 (2017). PubMed: 28108469
      • Helwig M  et al. Brain propagation of transduced a-synuclein involves non-fibrillar protein species and is enhanced in a-synuclein null mice. Brain 139:856-70 (2016). WB ; Mouse . PubMed: 26719384
      • Beyer K  et al. The decrease of {beta}-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies. Brain : (2010). WB ; Human . PubMed: 20959308
      • Iwata A  et al. alpha-Synuclein affects the MAPK pathway and accelerates cell death. J Biol Chem 276:45320-9 (2001). WB ; Human . PubMed: 11560921

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Synuclein antibody

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Brain, Cortex)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric Acid
      Permeabilization
      No
      Specification
      Brain, Cortex
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
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      Abcam user community

      Verified customer

      投稿 May 23 2018

      Question

      Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

      Please let me know if you require any further information.

      Looking forward for your response. When replying back can you please address the email “Dear Juli” as all replies come through a centralised email. This will avoid any confusion our end.

      Thanks

      Kind regards,

      Read More
      Answer

      Thank you for your enquiry regarding ab6165.

      I regret to inform you that the blocking peptide is not available commercially.

      Apologies!

      Read More

      Question

      Please see customers request below:

      Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

      Please let me know if you require any further information.

      Thanks

      Kind regards,

      Read More
      Answer

      Thank you for your email.

      I have contacted our laboratory and inquired about this.

      I will get back to you with the updates soon.

      Read More

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