Anti-beta III Tubulin (phospho S172) 抗体 (ab76286)
Key features and details
- Rabbit polyclonal to beta III Tubulin (phospho S172)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-beta III Tubulin (phospho S172) antibody
beta III Tubulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to beta III Tubulin (phospho S172) -
由来種
Rabbit -
特異性
The ab76286 sequence is identical to similar regions in bI, bII, and bIII tubulin isotypes. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: a wide range of other species -
免疫原
Synthetic peptide corresponding to Human beta III Tubulin (phospho S172) conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- C2C12 cells; purified brain tubulin treated with ERK2 kinase.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab76286 was cross adsorbed to unphosphorylated beta III Tubulin (Ser 172) peptide before affinity purification using phospho beta III Tubulin (Ser 172) peptide (without carrier). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76286の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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特記事項 |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
組織特異性
Expression is primarily restricted to central and peripheral nervous system. -
関連疾患
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- Unigene: 511743 Human
- Unigene: 40068 Mouse
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別名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
画像
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ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence.
Image 1: untreated.
Image 2: in the presence of the phospho peptide. -
All lanes : Anti-beta III Tubulin (phospho S172) antibody (ab76286) at 1/1000 dilution
Lane 1 : Purified human brain tubulin, untreated
Lane 2 : Purified human brain tubulin, treated with ERK2 kinase to phosphorylate
Ser 172
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab76286 は 4 報の論文で使用されています。
- Ge Z et al. The phosphatase of regenerating liver-3 protein (PRL-3) promotes glioma cell invasiveness by interacting with β3 -tubulin. Bioengineered 13:4112-4121 (2022). PubMed: 35098869
- Chang JH et al. Generation of Functional Dopaminergic Neurons from Reprogramming Fibroblasts by Nonviral-based Mesoporous Silica Nanoparticles. Sci Rep 8:11 (2018). PubMed: 29311646
- Ori-McKenney KM et al. Phosphorylation of ß-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis. Neuron 90:551-63 (2016). PubMed: 27112495
- Li WF et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). PubMed: 24728101