Application
Western blot
Sample
Mouse Cell lysate - whole cell (in vitro differentiated neural stem cell)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
5 µg
Specification
in vitro differentiated neural stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA/TBS
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pico
Negative control
Lane1- neural stem cell prior to the differentiation
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Positive control
Lane2 - in vitro differentiated neural stem cell lysate
Additional data
Additional Notes
Lane1- neural stem cell prior to the differentiation
Lane2 - in vitro differentiated neural stem cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 29 2015