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遺伝子ノックアウト細胞株 検証済

Anti-beta III Tubulin 抗体 - Neuronal Marker (ab18207)

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  • SDS
Reviews (65)Q&A (5)References (414)

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Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Flow Cytometry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Flow Cytometry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

Key features and details

  • Rabbit polyclonal to beta III Tubulin - Neuronal Marker
  • Suitable for: Flow Cyt, ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Common marmoset
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-beta III Tubulin antibody [EPR19591] (ab215037)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-beta III Tubulin antibody - Neuronal Marker
    beta III Tubulin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to beta III Tubulin - Neuronal Marker
  • 由来種

    Rabbit
  • 特異性

    The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. Please note that cross-reactivity with this protein has not been confirmed experimentally. TUB is localized notably in high concentrations in the nucleoli of brain neurons with lower protein levels in the cytoplasm. Please, therefore, be aware that ICC experiments may need to be optimised. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. As an alternative antibody, we would recommend our recombinant rabbit monoclonal antibody ab52623 which has been shown to specific in both WB and ICC using KO cells.
  • アプリケーション

    適用あり: Flow Cyt, ICC, IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Common marmoset, Dogfish, Catshark
    交差が予測される動物種: Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab18660)

  • ポジティブ・コントロール

    • Flow Cyt: Neuro 2A, U-87MG cells. IHC-P: Rat cerebellum, mouse brain, adult mouse ovaries, Dogfish/Catshark tissue (snout region). ICC: SK-N-SH cells, Neuro 2A, PC12 and RA induced P19 cells. Primary rat neurons/glia, DIV14. WB: WT HAP-1 whole cell lysate. Human, mouse and rat brain tissue lysate. Mouse hippocampus tissue lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Neuroscience
    • Development

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human beta III Tubulin peptide (ab18660)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Matched antibody pairs

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
  • Recombinant Protein

    • Recombinant Human beta III Tubulin protein (ab140581)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Venlafaxine hydrochloride, Serotonin-norepinephrine reuptake inhibitor (ab120715)
    • Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
    • Alexa Fluor® 647 Anti-beta III Tubulin antibody [2G10] (ab195880)
    • Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18207の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (3)
Use 0.01µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody

ICC (3)
Use a concentration of 1 µg/ml.
IHC-P (15)
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (10)
Use a concentration of 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta III Tubulin peptide (ab18660).
特記事項
Flow Cyt
Use 0.01µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody

ICC
Use a concentration of 1 µg/ml.
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta III Tubulin peptide (ab18660).

ターゲット情報

  • 機能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • 組織特異性

    Expression is primarily restricted to central and peripheral nervous system.
  • 関連疾患

    Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • 配列類似性

    Belongs to the tubulin family.
  • ドメイン

    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession Q13509 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 10381 Human
    • Entrez Gene: 22152 Mouse
    • Entrez Gene: 246118 Rat
    • Omim: 602661 Human
    • SwissProt: Q13509 Human
    • SwissProt: Q9ERD7 Mouse
    • SwissProt: Q4QRB4 Rat
    • Unigene: 511743 Human
    • Unigene: 40068 Mouse
    • Unigene: 43958 Rat
    see all
  • 別名

    • beta 3 tubulin antibody
    • beta 4 antibody
    • beta-4 antibody
    • CDCBM antibody
    • CDCBM1 antibody
    • CFEOM3 antibody
    • CFEOM3A antibody
    • FEOM3 antibody
    • M(beta)3 antibody
    • M(beta)6 antibody
    • MC1R antibody
    • Neuron specific beta III Tubulin antibody
    • Neuron-specific class III beta-tubulin antibody
    • QccE-11995 antibody
    • QccE-15186 antibody
    • TBB3_HUMAN antibody
    • Tubb 3 antibody
    • TUBB3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    • tuj 1 antibody
    • tuj1 antibody
    see all

画像

  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    ab18207 staining beta III Tubulin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18207 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    ab18207 staining beta III Tubulin in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18207 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Beta III Tubulin knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab18207 observed at 55 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab18207 was shown to recognize beta III Tubulin in wild-type HAP1 cells as signal was lost in beta III Tubulin knockout cells. An additional cross-reactive band at 50 kDa was observed in wild-type and knockout cells. Due to the immunogen’s homology with TUB (Tubby protein homolog, Uniprot: P50607), this lower band could correspond to the TUB protein. Please note that cross-reactivity with this protein has not been confirmed experimentally.

    Wild-type and beta III Tubulin knockout samples were subjected to SDS-PAGE. Ab18207 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature

  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    ab18207 staining beta III Tubulin in SK-N-SH (Human neuroblastoma cell line) cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab18207 at 1μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    IHC image of ab18207 staining beta III Tubulin in rat cerebellum formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18207, 1:2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Flow Cytometry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    Overlay histogram showing U-87MG (Human glioblastoma-astrocytoma epithelial cell line) cells stained with ab18207 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18207, 0.01μg/1x106) for 30 min at 22ºC. The secondary antibody used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/4000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in U-87MG cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    All lanes : Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human beta III Tubulin peptide (ab18660) at 2 µg/ml
    Lane 5 : Brain (Mouse) Tissue Lysate with Human beta III Tubulin peptide (ab18660) at 2 µg/ml
    Lane 6 : Brain (Rat) Tissue Lysate with Human beta III Tubulin peptide (ab18660) at 2 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    ab18207 staining beta III Tubulin in Neuro-2a cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab18207 at 1μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    ab18207 at 1/2000 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and an enzymatic antigen retrieval step was performed prior to incubation with the antibody for 16 hours. A biotinylated goat anti-rabbit IgG was used as the secondary.

    See Abreview

  • Flow Cytometry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Flow Cytometry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)

    Overlay histogram showing Neuro 2A cells stained with ab18207 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18207, 0.01μg/1x106) for 30 min at 22ºC. The secondary antibody used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/4000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (ab171870, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    All lanes : Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18207 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody , and visualised using ECL development solution ab133406

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    ab18207 at 1/2000 staining rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. A biotinylated goat polyclonal antibody was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for Neuron specific beta III Tubulin antibody - Neuronal Marker (ab18207) on Dogfish/Catshark Tissue sections (head: snout region). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/2000 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).

  • Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Western blot - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)This image is courtesy of an abreview submitted by Dr Sergi Bayod.
    All lanes : Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) at 1/1000 dilution

    All lanes : Mouse hippocampus tissue lysate

    Lysates/proteins at 8 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H&L) at 1/5000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds

    See Abreview

  • Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunocytochemistry - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)Sheikh, M.A. et al PLoS One. 2013;8(2):e55826. doi: 10.1371/journal.pone.0055826. Epub 2013 Feb 7 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Differential expression of Dnmt1, Dnmt3a, and Dnmt3b during RA induced neuronal differentiation of P19 cells

    Mouse P19 cells either left untreated (top panel) or RA treated for initial 2 days and further cultured for 4 days without RA (6 days, bottom panel) were immunostained with neuron specific β-III tubulin antibody and nuclei were stained using DAPI.

    In order to confirm the neuronal morphology, the cells were stained for neuron specific beta III-tubulin (ab18207). RA induced P19 cells showed immunoreactivity against βIII-tubulin, indicating a neuronal phenotype. In contrast, undifferentiated P19 cells were βIII-tubulin negative.

    (After Figure 1A of Sheikh et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)Courtesy of Feng Y et al. Sci Rep. 2017; 7: 44810. doi: 10.1038/srep44810 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemical analysis of adult mice ovaries undergone Clarity processing staining tyrosine hydroxlase (TH), Beta III Tubulin (Tuj1) with ab18207, and brain derived neurotrpic factor (BDNF) with ab72439. Positive staing of Tuj1 and BDNF is evident in the theca cells and corpus luteum.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (414)

ab18207 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18207 は 414 報の論文で使用されています。

  • Zhang H  et al. miR-34a-5p up-regulates the IL-1ß/COX2/PGE2 inflammation pathway and induces the release of CGRP via inhibition of SIRT1 in rat trigeminal ganglion neurons. FEBS Open Bio 11:300-311 (2021). PubMed: 33155431
  • Mansor NI  et al. Asymptomatic neurotoxicity of amyloid ß-peptides (Aß1-42 and Aß25-35) on mouse embryonic stem cell-derived neural cells. Bosn J Basic Med Sci 21:98-110 (2021). PubMed: 32156249
  • Deng CL  et al. Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration. Cell Death Differ 28:1041-1061 (2021). PubMed: 33082517
  • Wan T  et al. Increased telomerase improves motor function and alpha-synuclein pathology in a transgenic mouse model of Parkinson's disease associated with enhanced autophagy. Prog Neurobiol 199:101953 (2021). PubMed: 33188884
  • Solomon EA  et al. Neuromuscular Junction Model Optimized for Electrical Platforms. Tissue Eng Part C Methods 27:242-252 (2021). PubMed: 33599165
View all Publications for this product

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1-10 of 70 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
Specification
mouse brain
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Paraformaldehyde
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MR. Lenin Veeraval

Verified customer

投稿 Aug 11 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse brain)
Specification
mouse brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Paraformaldehyde
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MR. Lenin Veeraval

Verified customer

投稿 Aug 10 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal spheroid)
Permeabilization
Yes - 1% Triton-X
Specification
Hippocampal spheroid
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Mar 19 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cortical spheroid)
Permeabilization
Yes - 1% Triton-X
Specification
Cortical spheroid
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Mar 19 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Stem cell)
Permeabilization
Yes - 1% Triton-X
Specification
Stem cell
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Mar 19 2021

IHC - Wholemount abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Cortical)
Specification
Cortical
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Abcam user community

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投稿 Aug 11 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hippocampus)
Permeabilization
Yes - TritonX
Specification
Hippocampus
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 11 2020

Immunocytochemistry abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cortical)
Permeabilization
Yes - TritonX
Specification
Cortical
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

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投稿 Aug 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tooth)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton
Specification
tooth
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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DR. Tina Yuan

Verified customer

投稿 Sep 30 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta III Tubulin antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Oct 24 2018

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