Alexa Fluor® 555 Anti-beta III Tubulin 抗体 [EP1569Y] - Neuronal Marker (ab202519)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EP1569Y] to beta III Tubulin - Neuronal Marker
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
製品の概要
-
製品名
Alexa Fluor® 555 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker
beta III Tubulin 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 555 Rabbit monoclonal [EP1569Y] to beta III Tubulin - Neuronal Marker -
標識
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- ICC/IF: U87MG cells, HAP1 cells (HAP1-TUBB3 knockout cells used as negative cell line)
-
特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1569Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190574)
- Alexa Fluor® 647 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab190575)
- Biotin Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab195903)
- Alexa Fluor® 594 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab201740)
- Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
- APC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224977)
- FITC Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224978)
- PE Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab224979)
- Alexa Fluor® 488 Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab283728)
- Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (ab52623)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab202519の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/200 - 1/500.
This product gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
特記事項 |
---|
ICC/IF
1/200 - 1/500. This product gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
ターゲット情報
-
機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
組織特異性
Expression is primarily restricted to central and peripheral nervous system. -
関連疾患
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
-
参照データベース
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Entrez Gene: 246118 Rat
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- SwissProt: Q4QRB4 Rat
- Unigene: 511743 Human
see all -
別名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
画像
-
ab202519 staining beta III tubulin in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202519 at a 1/500 dilution (shown in green) and ab195884, Rat monoclonal [YOL1/34] to Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
ab202519 staining beta III Tubulin in U87MG cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202519 at 1/200 dilution (shown in yellow). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in U87MG cells fixed with 100% methanol (5min).
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (2)
ab202519 は 2 報の論文で使用されています。
- Kamynina A et al. Activation of RAGE leads to the release of glutamate from astrocytes and stimulates calcium signal in neurons. J Cell Physiol 236:6496-6506 (2021). PubMed: 33570767
- Knight VB & Serrano EE Post-Translational Tubulin Modifications in Human Astrocyte Cultures. Neurochem Res 42:2566-2576 (2017). PubMed: 28512712