Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1569Y] to beta III Tubulin (Alexa Fluor® 594)
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
製品名Anti-beta III Tubulin antibody [EP1569Y] (Alexa Fluor® 594)
beta III Tubulin 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EP1569Y] to beta III Tubulin (Alexa Fluor® 594)
標識Alexa Fluor® 594. Ex: 590nm, Em: 617nm
アプリケーション適用あり: ICC/IFmore details
種交差性交差種: Rat, Human
交差が予測される動物種: Mouse, Zebrafish
Synthetic peptide within Human beta III Tubulin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13509
- ICC/IF: PC12 cells treated with NGF, HAP1 cells (HAP1-TUBB3 knockout cells used as negative cell line)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
精製度Protein A purified
- Anti-beta III Tubulin antibody [EP1569Y] (HRP) (ab190574)
- Anti-beta III Tubulin antibody [EP1569Y] (Alexa Fluor® 647) (ab190575)
- Anti-beta III Tubulin antibody [EP1569Y] (Biotin) (ab195903)
- Anti-beta III Tubulin antibody [EP1569Y] (Alexa Fluor® 568) (ab201849)
- Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (Alexa Fluor® 555) (ab202519)
- Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
- Anti-beta III Tubulin antibody [EP1569Y] (APC) (ab224977)
- Anti-beta III Tubulin antibody [EP1569Y] (FITC) (ab224978)
- Anti-beta III Tubulin antibody [EP1569Y] (PE) (ab224979)
- Anti-beta III Tubulin antibody [EP1569Y] (ab52623)
Our Abpromise guarantee covers the use of ab201740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
組織特異性Expression is primarily restricted to central and peripheral nervous system.
関連疾患Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
配列類似性Belongs to the tubulin family.
ドメインThe highly acidic C-terminal region may bind cations such as calcium.
翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
細胞内局在Cytoplasm > cytoskeleton.
- Information by UniProt
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
ab201740 staining beta III Tubulin in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab201740 at 1/500 dilution (shown in red) and ab195887at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab201740 staining beta III Tubulin in PC12 cells treated with NGF. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201740 at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in PC12 cells fixed with 100% methanol (5 min).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201740 は 1 報の論文で使用されています。
- Batista AFR et al. Intra-axonal Synthesis of SNAP25 Is Required for the Formation of Presynaptic Terminals. Cell Rep 20:3085-3098 (2017). PubMed: 28954226