Western blot abreview for Anti-beta III Tubulin antibody [EP1569Y]

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS, Tween 0.5%, Milk 5%, NaN3
Dilution
1/4000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Clarity
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

Additional data

Additional Notes
Lane 1: Ladder Lane 2: Human intestinal tissue lysate (mucosa and submucosa)

Mr. Julien Chevalier

Verified customer

投稿 Sep 30 2015

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