Immunocytochemistry/ Immunofluorescence abreview for Anti-beta III Tubulin antibody [EP1569Y]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Specification
Cryopreserved embryonic cortical neurons (QBMcells
Permeabilization
No
Fixative
paraformaldehyde with picric acid

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10mM PBS with 0.3% Triton-X
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit AlexaFluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
QBM Cell Science cryopreserved rat cortical neurons cultured for 7 days on poly-D-lysine coated 96 well plates. Good antibody. 1:50 was better on these very dense cultures but 1:100 worked well also, just a little less intense. Results were as expected.

Ms. Babben Tinner

Verified customer

投稿 Feb 26 2015

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