Application
Immunohistochemistry (Frozen sections)
Sample
Lizard Tissue sections (Anolis carolinensis regenerated tail spinal cord c)
Permeabilization
Yes - 0.1% Triton X-100/TBS for 10 min at room temperature
Specification
Anolis carolinensis regenerated tail spinal cord c
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in 0.1% Tween20/TBS
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: ThermoFisher Goat Anti-Rabbit Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
16 µm sections of tissues fixed overnight in PFA at 4°C. Sections were blocked for 1 hour at room temperature in 1% BSA in 0.1% Tween20/TBS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Megan Hudnall
Verified customer
投稿 Mar 20 2020