製品の概要

  • 製品名
    Anti-beta Galactosidase antibody [DC1 4C7]
    beta Galactosidase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DC1 4C7] to beta Galactosidase
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 免疫原

    beta Galactosidase EC.3.2.1.23 from E.coli.

  • 特記事項

    This monoclonal is also known as clone HD4C7 or HD 447C.

    This antibody clone is manufactured by Abcam. If you require a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    DC1 4C7
  • ミエローマ
    P3-NS1/1-Ag4-1
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 細胞内局在
    Cytoplasmic
  • 別名
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

画像

  • All lanes : Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg

    Lane 1 : Recombinant Human GLB1 protein (ab151890)
    Lane 2 : beta Galactosidase protein (ab79449)

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time: 4 minutes

プロトコール

参考文献

This product has been referenced in:
  • Smith SJ  et al. Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition. PLoS One 7:e52335 (2012). IHC-P . Read more (PubMed: 23272238) »
  • Nassiri M  et al. Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice. Arterioscler Thromb Vasc Biol 30:1423-9 (2010). IHC-Fr . Read more (PubMed: 20431063) »
See all 5 Publications for this product

レビューと Q&A

1-10 of 13 Abreviews or Q&A

Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We do not have a recommended dilution of this antibody. The dilution employed will depend on the level of expression of your LacZ construct. However, I would certainly expect detection of LacZ in view of your detection of His and detection of your construct using an anti-His antibody. Can you tell me whether you are detecting whole LacZ tagged with His as I would like to confirm that the epitope is present on the construct.

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Question

BATCH NUMBER 286 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal in all samples SAMPLE 1. ?-gal fusion protein from engineered ES cell 2. Over-expressed ?-gal 3. Purified ?-gal protein PRIMARY ANTIBODY Abcam / Mouse / 3% BSA / 1:2000, 1000, 500 or 100 / 1 hour at RT or overnight at 4?, 4 x 5 min wash with PBS-T DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Positive controls : Purified ?-gal protein, ?-gal transfected cell lysate Negative control : not transfected cell lysate ANTIBODY STORAGE CONDITIONS -70? / No reconstitution SAMPLE PREPARATION 1X SDS gel loading buffer AMOUNT OF PROTEIN LOADED 10 ng for purified protein, 60 ?g for lysates ELECTROPHORESIS/GEL CONDITIONS Reducing, 6% or 7% gel TRANSFER AND BLOCKING CONDITIONS 25 mM Tris base, 192 mM glycine, 20% methanol / 2 hours, 5% BSA or 10% skim milk in PBS-T (0.1% Tween-20) SECONDARY ANTIBODY Jackson InmunoResearch / Donkey / 3% BSA / 1:10000, 5000 / 1 hour at RT, 4 x 5 min wash with PBS-T HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 10 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Antibody dilution factor, incubating condition ADDITIONAL NOTES ? We want to replace not-working monoclonal ab(ab116) with polyclonal anti-?-gal antibody(Abcam, Cat#. ab616) We guarantee our samples and immunoblotting conditions because we successfully detected endogenous Bmal1 protein(our major target) from the same blots.

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Answer

Thank you for your enquiry and for submitting your technical questionnaire. I am sorry to hear that your customer has been having difficulties with this antibody. I have read their technical enquiry and they have tried some of the recommendations that I would make such as reducing the concentration of the antibody or blocking in BSA. However, I would be interested to see the blot image and information regarding whether your customer can be certain that beta-gal is being expressed to a significant level by these cells. An enzymatic detection of beta-gal by its substrate will be significantly more sensitive than detection of the protein itself by western blotting. I understand that your customer has been using a purified protein preparation of beta-gal. However, it may be that the antibody's sensitivity cannot detect levels as low as 10ng. I appreciate the patience of your customer in this matter and look forward to hearing from you.

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Answer

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We would like to confirm that this antibody has not been tested for immunohistochemistry, although it is suitable for IF and ICC. So you may need to fix the sample in a more gentle way, we would advise to fix the samples for shorter time (15 to 30 min) than you would normally do with formalin-fixed tissue sections. We have another alternative antibody ab616 which has been tested for IHC both on frozen and formalin-fixed sections and it is one of our best sellers. We have also received very good feedback information from our customers and published 15 reviews on-line. We hope this information will be useful for you.

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Answer

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please do let us know.

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Answer

Yes, ab616 has successfully been tested for application in IHC-formalin fixed paraffin embedded sections. We have had some feedback from customers who have used ab616 in IHC-P to detect beta gal in transgenic mice - you may read their comments by clicking on the reviews tab located on the online datasheet. We also have a specific IHC protocol for this antibody - the protocol can be found in the protocols tab on the online datasheet. If you have any additional questions, please do contact us again.

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Answer

Thank you for your email. Neither ab116 or ab1047 have been tested yet for application in IHC. You may want to look at ab616 which has been tested in IHC. If you decide to try ab116 or ab1047 in IHC, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. However, because ab116 and ab1047 have not yet been tested in IHC we are unable to provide a guarantee. If you have any additional questions, please contact us again.

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Question
Answer

This anitbody has not yet been tested in immunocytochemistry

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Question
Answer

Yes this antibody does work in this application (although there maybe some post-translational modification of the b-gal fusion protein which may decrease the binding of the antibody)

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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