Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Specification
retina
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 2% serum 0.2% Triton-X
Secondary antibody
Name
Non-Abcam antibody was used: invitrogen goat anti-chicken Alexa fluor488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
PubMed ID
19793992: View
Additional Notes
Antibody works very well for ICC on cryostat sections of OCT emebedded tissues.
Sucessfully labels B-gal expressing cells in transgenic reporter mice at dilutions of ~1:1500.
Sucessfully labels B-gal expressing cells in transgenic reporter mice at dilutions of ~1:1500.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. steven hughes
Verified customer
投稿 Mar 05 2010