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  1. Link

    beta-galactosidase-antibody-ab9361.pdf

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Tags & Cell Markers Fusion / Marker Proteins Beta Galactosidase
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Anti-beta Galactosidase 抗体 (ab9361)

  • Datasheet
  • SDS
Reviews (36)Q&A (50)References (475)

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Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)
  • Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)

Key features and details

  • Chicken polyclonal to beta Galactosidase
  • Suitable for: IHC-FrFl, IHC-FoFr, ICC/IF, IHC-Fr, IHC-P
  • Reacts with: Escherichia coli
  • Isotype: IgY

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-beta Galactosidase antibody [13R4] (ab206546)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-beta Galactosidase antibody
    beta Galactosidase 一次抗体 製品一覧
  • 製品の詳細

    Chicken polyclonal to beta Galactosidase
  • 由来種

    Chicken
  • アプリケーション

    適用あり: IHC-FrFl, IHC-FoFr, ICC/IF, IHC-Fr, IHC-Pmore details
  • 種交差性

    交差種: Escherichia coli
  • 免疫原

    Full length native protein (purified). The immunogen was purified beta-galactosidase from Escherichia coli.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgY
  • 研究分野

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Beta Galactosidase

関連製品

  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Native E. coli beta Galactosidase protein (ab79449)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9361の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FrFl (4)
Use at an assay dependent concentration.
IHC-FoFr (4)
1/1000 - 1/2000.
ICC/IF (9)
Use at an assay dependent concentration.
IHC-Fr (9)
Use at an assay dependent concentration.
IHC-P (7)
1/200 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-FrFl
Use at an assay dependent concentration.
IHC-FoFr
1/1000 - 1/2000.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/200 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 細胞内局在

    Cytoplasmic
  • 参照データベース

    • Entrez Gene: 945006 Escherichia coli
    • SwissProt: B7UJI9 Escherichia coli
    • SwissProt: P00722 Escherichia coli
    • 別名

      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)
      Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)Image from PLoS One. 2013; 8(11): e77928, Fig 2, doi: 10.1371/journal.pone.0077928 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      P0-adult mice were euthanized and perfused with 4% paraformaldehyde in PBS (PF). Their spinal cords were then post-fixed for 30–60 mins in 4% PF at 4°C (P0) or at room temperature (adult). Spinal cords were rinsed and cryoprotected in 20% sucrose in PBS (4°C) prior to embedding in OCT (Tissue-Tek). Immunostaining of frozen spinal sections was performed by incubating 20 µm thick sections with primary antibodies, which were then detected using species-specific secondary antibodies conjugated with Cy2, Cy3 and Cy5 or FITC. ab9361 was used at 1:1000.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)This image is courtesy of an Abreview submitted by Laurel Baglia

      ab9361 staining beta Galactosidase in mouse e13 stomach and liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Davidson's fixative, permeabilized with 0.5% Triton-X 100 and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBST + 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)
      Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)Image courtesy of Dr Sean Speese by Abreview.
      ab9361 staining beta Galactosidase in fruit fly central nervous system glia cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton. Samples were then incubated with primary antibody at 1/2000 for 12 hours at 4°C. The secondary antibody used was a donkey anti-chicken monoclonal conjugated to DyLight® 649 (pink) used at a 1/400 dilution. Nuclei stained with a pan nuclear marker (green).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Galactosidase antibody (ab9361)Image from PLoS One. 2013; 8(11): e80801, Fig 1B, doi: 10.1371/journal.pone.0080801 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

      Ab9361 staining Beta galactosidase in Mouse thyroid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed 10% buffered formalin, cut into 3-4 micron slices, blocked with 10% normal goat serum and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with the following primary antibodies; anti-BrdU, anti-Sca1 (ab109211, 1∶600), anti-NKX2-1 and anti-β-gal (ab9361, 1∶1000). Sections were incubated with the first primary antibody (anti-Sca1) for 1 hour at room temperature. After washing with PBS, sections were incubated with the first secondary antibody (Alexa Fluor 555 goat anti-rabbit IgG) and washed with PBS. Sections were then incubated with normal serum (5% rabbit serum) from the same host species as the first primary antibodies for 1 hour at room temperature and washed with PBS. Sections were further incubated with an excess of unconjugated Fab antibody derived from the same host species as the primary antibody for 1 hour at room temperature and washed with PBS. The sections were finally incubated with the mixed second primary antibodies (anti-BrdU, anti-β-gal, anti-NKX2-1) overnight at 4°C, washed with PBS, and were incubated with the second secondary antibody (Alexa Fluor 488 goat anti-rat IgG, Dylight 650 goat anti-chicken IgY, Dylight 594 goat anti-rabbit IgG) for 1 hour at room temperature and washed with PBS. DAPI dye was used to stain the nuclei of cells.

    • Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)
      Immunohistochemistry (Frozen sections) - Anti-beta Galactosidase antibody (ab9361)This image is courtesy of an Abreview submitted by Dr Steven Hughes

      ab9361 staining Beta Galactosidase in mouse retinal tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.2% triton-X and blocked with 5% serum for 1 hour at 23°C. The sample was incubated with primary antibody (1/1500 in PBS, 2% serum, 0.2% Triton-X) at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat monoclonal to chicken IgY (1/200) was used as secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)
      Immunocytochemistry/ Immunofluorescence - Anti-beta Galactosidase antibody (ab9361)This image is courtesy of an Abreview submitted by Dr Deon Wolpowitz
      ab9361 at 1/250 staining human HEK293T cells by ICC/IF. The cell line was transfected with a b-gal expressing plasmid, and x-gal staining was performed on adjacent wells. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. A Texas Red conjugated donkey anti-chicken antibody was used as the secondary.

      See Abreview

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (475)

    ab9361 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9361 は 475 報の論文で使用されています。

    • Marneros AG Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron. Cell Rep 34:108616 (2021). PubMed: 33440155
    • Chatzeli L  et al. Comparing development and regeneration in the submandibular gland highlights distinct mechanisms. J Anat 238:1371-1385 (2021). PubMed: 33455001
    • Jing J  et al. Reciprocal interaction between mesenchymal stem cells and transit amplifying cells regulates tissue homeostasis. Elife 10:N/A (2021). PubMed: 33480845
    • Liang X  et al. The SNAG Domain of Insm1 Regulates Pancreatic Endocrine Cell Differentiation and Represses ß- to d-Cell Transdifferentiation. Diabetes 70:1084-1097 (2021). PubMed: 33547047
    • Lowenstein ED  et al. Olig3 regulates early cerebellar development. Elife 10:N/A (2021). PubMed: 33591268
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 86 Abreviews or Q&A

    Immunohistochemistry - Free Floating abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 12 2016

    Immunohistochemistry free floating abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Escherichia coli Tissue sections (Mouse Brain)
    Specification
    Mouse Brain
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 17 2021

    Western blot abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Tissue lysate - whole (Mouse Brain)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    50 µg
    Specification
    Mouse Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 16 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Escherichia coli Tissue sections (Mouse Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS 1x, Triton x-100 1%
    Specification
    Mouse Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 16 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (18dpc ShhcreErt2/+R26R-LacZ Male Urogenital Sinus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
    Permeabilization
    No
    Specification
    18dpc ShhcreErt2/+R26R-LacZ Male Urogenital Sinus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 22 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (E12.5 Embryo)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EGTA, pH=9, pressure cooker
    Permeabilization
    Yes - Saponi
    Specification
    E12.5 Embryo
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 16 2016

    Immunohistochemistry - Free Floating abreview for Anti-beta Galactosidase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 14 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Galactosidase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Larval brain)
    Permeabilization
    Yes - TBS-T
    Specification
    Larval brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Intestine)
    Permeabilization
    Yes - .01 SDS
    Specification
    Intestine
    Blocking step
    Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 22 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Galactosidase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Main Olfactory Epithelium (MOE))
    Permeabilization
    Yes - TritonX 0.01%
    Specification
    Main Olfactory Epithelium (MOE)
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Jennifer Cisson

    Verified customer

    投稿 May 11 2015

    1-10 of 86 Abreviews or Q&A

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