製品の概要
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製品名Anti-beta Galactosidase antibody
beta Galactosidase 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to beta Galactosidase
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由来種Rabbit
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アプリケーション適用あり: ELISA, WB, IPmore details
適用なし: ICC/IF,IHC-FoFr,IHC-Fr or IHC-P -
種交差性交差種: Escherichia coli
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免疫原
Recombinant full length protein (E. coli).
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ポジティブ･コントロール
- 1.0µg of E.coli in a standard sandwich ELISA. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified E.coli beta-galactosidase.
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特記事項
Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度IgG fraction
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特記事項（精製）IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| ELISA | 1/10000. sELISA tested against 1ug E.coli beta galactosidase. |
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| WB | 1/5000 - 1/10000. Detects a band of approximately 116 kDa. This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives. |
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| IP | 1/500 - 1/5000. |
ターゲット情報
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関連性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
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細胞内局在Cytoplasmic
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参照データベース
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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別名
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
画像
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Western blot - Anti-beta Galactosidase antibody (ab616)Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.
参考文献
This product has been referenced in:
- Chen ZJ et al. Renoprotective Effect of a Chinese Herbal Formula, Qidan Dihuang Decoction, on Streptozotocin-Induced Diabetes in Rat. Evid Based Complement Alternat Med 2018:7321086 (2018). Read more (PubMed: 29849726) »
- Chodelkova O et al. Tcf7L2 is essential for neurogenesis in the developing mouse neocortex. Neural Dev 13:8 (2018). IHC-P . Read more (PubMed: 29751817) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"