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    beta-galactosidase-antibody-ab616.pdf

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Tags & Cell Markers Fusion / Marker Proteins Beta Galactosidase
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Anti-beta Galactosidase 抗体 (ab616)

  • Datasheet
  • SDS
Reviews (30)Q&A (44)References (109)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-beta Galactosidase antibody (ab616)

    Key features and details

    • Rabbit polyclonal to beta Galactosidase
    • Suitable for: ELISA, WB, IP
    • Reacts with: Escherichia coli
    • Isotype: IgG

    こちらの製品もご検討ください

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    関連製品

    製品の概要

    • 製品名

      Anti-beta Galactosidase antibody
      beta Galactosidase 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to beta Galactosidase
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: ELISA, WB, IPmore details
      適用なし: ICC/IF,IHC-Fr or IHC-P
    • 種交差性

      交差種: Escherichia coli
    • 免疫原

      Recombinant full length protein (E. coli).

    • 特記事項

      Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...
    • 精製度

      IgG fraction
    • 特記事項(精製)

      IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • Beta Galactosidase

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Native E. coli beta Galactosidase protein (Active) (ab7981)
    • Recombinant Protein

      • Native E. coli beta Galactosidase protein (ab79449)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab616の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    1/10000.

    sELISA tested against 1ug E.coli beta galactosidase.

    WB (8)
    1/5000 - 1/10000. Detects a band of approximately 116 kDa.

    This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives.

    IP
    1/500 - 1/5000.
    特記事項
    ELISA
    1/10000.

    sELISA tested against 1ug E.coli beta galactosidase.

    WB
    1/5000 - 1/10000. Detects a band of approximately 116 kDa.

    This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives.

    IP
    1/500 - 1/5000.
  • 追加情報
    Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
  • ターゲット情報

    • 関連性

      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • 細胞内局在

      Cytoplasmic
    • 参照データベース

      • Entrez Gene: 945006 Escherichia coli
      • SwissProt: B7UJI9 Escherichia coli
      • SwissProt: P00722 Escherichia coli
      • 別名

        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      画像

      • Western blot - Anti-beta Galactosidase antibody (ab616)
        Western blot - Anti-beta Galactosidase antibody (ab616)
        All lanes : Anti-beta Galactosidase antibody (ab616)

        Lanes 1-2 : Non-reduced purified recombinant beta Galactosidase protein
        Lanes 3-4 : reduced purified recombinant beta Galactosidase protein


        Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.

      プロトコール

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (109)

      ab616 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab616 は 109 報の論文で使用されています。

      • Péladeau C  et al. Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles. Nat Commun 11:1990 (2020). PubMed: 32332749
      • Qiao W  et al. 1,25-Dihydroxyvitamin D insufficiency accelerates age-related bone loss by increasing oxidative stress and cell senescence. Am J Transl Res 12:507-518 (2020). PubMed: 32194899
      • Hilbold E  et al. Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch. Cells 9:N/A (2020). PubMed: 32164318
      • de Vreede G  et al. A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation. Dev Cell 45:595-605.e4 (2018). PubMed: 29870719
      • Park H  et al. Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms. Nat Commun 9:5293 (2018). PubMed: 30546048
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 74 Abreviews or Q&A

      Question

      Thank you very much for the discount offers.

      We will order the following:

      beta-galactosidase Ab: ab616

      Ki67 Ab: ab15580

      ICE support pack: ab111542

      Read More

      Abcam community

      Verified customer

      Asked on Jun 28 2012

      Answer

      I am very pleased to hear you would like to accept our offer and testab616 and ab15580in ICE. These codes will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for ICE for each antibody and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jun 28 2012

      Western blot

      Excellent
      Abreviews
      Abreviews
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (epidermis)
      Specification
      epidermis
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      投稿 May 29 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate
      Sample
      Mouse Tissue sections (epidermis)
      Specification
      epidermis
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 May 28 2013

      Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Galactosidase antibody

      Good
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (ROSA LacZ/+ AMH-Cre adult testes)
      Specification
      ROSA LacZ/+ AMH-Cre adult testes
      Fixative
      4 ? Acetone:Methanol 1:1
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. Qing Wen

      Verified customer

      投稿 Dec 20 2012

      Question


      Nous souhaiterions faire des immunomarquages pour beta-galactosidase sur
      coupes paraffine. Nous souhaiterions donc acheter un anticorps. Nous
      serions éventuellement intéressé par l'anticorps Ab616 de votre catalogue mais nous avons quelques doutes. En effet, sur le site
      Internet il est seulement préconisé pour ELISA, IP et WB, mais dans la
      fiche technique, il y a quelques indications pour immunohistochimie.
      Afin de le tester dans notre laboratoire avant achat, disposeriez vous
      d'un aliquot ou le vendez vous en plus petite quantité ?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 17 2012

      Answer

      Merci de nous avoir contactés.

      J'ai pu voir d'après nos archives que plusieurs utilisateurs ont rencontré des problèmes avec cet anticorps en IHC-P. L'application IHC-P a d'ailleurs été retirée de la liste des applications testées, mais malheureusement pas l'image que vous avez mentionnée; ceci est une erreur de notre part que je vais signaler à mes collègues responsables des fiches techniques.

      De plus, nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, je peux vous recommander d’utiliser l’anticorps ab4761 qui a été testé en IHC-P et est couvert par notre Garantie Abpromise. Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique (espèces et applications), nous offrons un remboursement ou un remplacement gratuit (https://www.abcam.com/abpromise).


      J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

      Read More

      Abcam Scientific Support

      Answered on Oct 17 2012

      Question

      ReviewID: xxx AbID: 616, Anti-beta Galactosidase antibody Rating: Inconclusive Sample: Species: Human Type: Cell Specification: H407 cells Application: Application: Blocking step: AbCam ICE kit as blocking agent for 2 hours at 37°C Type:: Indirect Other detail: Dilution: 1/500 Incubation time: 24 hours at 4°C Diluent: from ICE kit Secondary Antibody: Name: Non-Abcam Antibody was used: goat anti-rabbit IgG-HRP Conjugation: HRP Additional Data: Additional Notes: Senescence of H407 was induced by Toxin up to 7 days. Beta-galactosidase activity was detected by X-Gal based staining kit. But this antibody does not detect beta-galactosidase on IF and ICE.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 10 2012

      Answer

      Thank you for submitting an Abreview for ab616. As you may have noticed, your review has now been published on our website.
      Since you obtained poor results using the antibody in an untested species, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
      Based on the information in your review, I have a few more questions:
      1) Did you use the antibody to detect human beta Galactosidase ?
      2) If not, for which application or species was the discount code given?
      In terms of suggestions:
      - It might be helpful to compare the resuts obtained with human beta Galactosidase with E.coli beta Galactosidase.
      If you decide to follow these suggestions, please let me know if they are helpful.
      I wish you good luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Oct 10 2012

      ELISA abreview for Anti-beta Galactosidase antibody

      Inconclusive
      Abreviews
      Abreviews
      Application
      ELISA
      Sample
      Human Cell (H407 cells)
      Specification
      H407 cells
      Blocking step
      AbCam ICE kit as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
      Type
      Indirect
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 10 2012

      Question

      Phone call requesting information in regards to the Anti-beta Galactosidase antibody (ab616) and the beta Galactosidase protein (ab7981).

      Read More

      Abcam community

      Verified customer

      Asked on Jul 04 2012

      Answer

      Thank you for contacting us yesterday.

      I am sorry to hear you are experiencing difficulties with one of our products. As discussed over the phone, I'd like to try and help you to improve the results currently obtained. In order to do this, could you please fill out the questionnair I have attached to this email. If you could also include some images of the blots obtained that would be very helpful. I understand that you have already performed a lotof optimisation but if you could just briefly outline what you have tried to optimise that would be very helpful.

      I think it would be worthwhile to try and use the beta Galactosidase protein (ab7981) which you have just to check that there is not a problem with the antibody itself thatare contributing to the poor results.

      In answer to you questions in regards to how to use this protein as a control. We recommend reconstituting the protein in a minimum concentration of 1 mg/mL in 0.1 M Sodium/potassium phosphate buffer, pH 7.0. This can be performed as follows:

      0.1 M Sodium phosphate buffer: Dissolve 1.42 gms sodium phosphate dibasic (MW 141.98) in reagent grade water to a final volume of 100 ml.

      0.1 M Potassium phosphate buffer: Prepare by dissolving 1.36 gms potassium phosphate monobasic (MW 136.1) in reagent grade water to a final volume of 100 ml.

      0.1 M Sodium/potassium phosphate, pH 7.0: Take 60 ml sodium phosphate buffer 0.1 M and adjust pH to 7.0 with 0.1 M potassium phosphate buffer. Buffer may be stored at 2 - 8°C for 2 - 3 weeks.

      The lypolised powder the protein is provided as can be stored at2-8°C but once dissolved it is best to aliquot and freeze stocks at -20C or -80C. We do not have any reconstituted stability data andwould therefore recommend to reconstitute the amount needed for the assay fresh just prior to performing the assay.

      I would recommend initially loading 0.1 ug in a well to see how well the antibody performs next to your samples. This value may need to be reduced if the signal observed is too strong compared to your lysates.

      I hope this information has been of help. I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on Jul 04 2012

      Question

      Sehr geehrte Damen und Herren,

      für unsere Experimente wären sehr an einem funktionierenden ß-Gal AB interresiert. Leider haben wir schon mit über 4 Antikörper von verschiedenen
      Herstellern schlechte Erfahrungen gemacht.

      Durch folgende Veröffentlichung wurden wir auch Ihren AB aufmerksam:

      Sonic Hedgehog Signaling Mediates Epithelial–Mesenchymal Communication and Promotes Renal Fibrosis JASN May 1, 2012 23: 801-813; published ahead of print February 2, 2012, doi:10.1681/ASN.2011060614
      Sehr gerne würde wir Ihren Antikörper (ab616) testen, falls dies möglich ist. Könnten Sie uns ein Batch für einige Testfärbungen zur Verfügung stellen?

      Mit freundlichen Grüßen,


      --

      Read More

      Abcam community

      Verified customer

      Asked on May 16 2012

      Answer

      Vielen Dank für Ihre Anfrage.

      Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird, können wir leider keinen Ersatz anbieten, wir möchten Sie jedoch einladen unser Abreview-System zu verwenden.

      Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.

      Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren:

      https://www.abcam.com/abreviews

      Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

      Read More

      Abcam Scientific Support

      Answered on May 16 2012

      Question

      Bonjour, Monsieur
      Pour l'instant, j'essaie juste de faire marcher un contrôle positif: un autre facteur de transcription avec la même colocalisation. Et le marquage ne marche que si je mets du Triton 0.1 dans tous les milieu d'incubation, mais je perd le contre-marquage membrannaire qui me permet d'identifier les cellules d'intérêt. Je suis en train de substituer le triton avec du Tween dans différentes étapes. Quand j'aurai un beau contrôle positif, je re-testerai l'AC d'abcam et vous tiendrai au courrant…. Mais ça risque deprendre un peu de temps.
      Merci en tout cas pour votre disponnibilité.
      Bien cordialement,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 09 2012

      Answer

      Merci pour ces précisions.

      Je vous souhaite une bonne continuation dans vos expérimentations. N'hésitez pas à nous contacter de nouveau si vous avez des questions.

      Read More

      Abcam Scientific Support

      Answered on Mar 09 2012

      1-10 of 74 Abreviews or Q&A

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      For licensing inquiries, please contact partnerships@abcam.com

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