Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Embryo (Whole embryos. Formeldahyde fixed.)
Specification
Whole embryos. Formeldahyde fixed.
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBX
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 IgY (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Embryos were fixed in 4% Formaldehyde.
Dilutions 1:1000, 1:5000, and 1:10,000 were tested. While all dilutions worked, 1:5000 seemed optimal.
The attached image is 1:10,000 dilution. Red-Bgal (BAD-lacZ, enhancer of Rhomboid), Green is 22C10 (peripheral nervous system marker)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 27 2016