Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-beta Galactosidase 抗体 (ab12081)

Reviews (1)Q&A (3)References (6)
Western blot - Anti-beta Galactosidase antibody (ab12081)

    Key features and details

    • Goat polyclonal to beta Galactosidase
    • Suitable for: WB
    • Reacts with: Escherichia coli
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-beta Galactosidase antibody [13R4] (ab206546)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-beta Galactosidase antibody
      beta Galactosidase 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to beta Galactosidase

    • 由来種

      Goat

    • 特異性

      Detects recombinant beta galactosidase enzyme by Western blotting.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Escherichia coli

    • 免疫原

      Recombinant full length protein corresponding to Escherichia coli beta Galactosidase.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.40
      Preservative: 0.025% Sodium azide
      Constituent: PBS

      This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab12081の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.
    特記事項
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.

    ターゲット情報

    • 関連性

      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

    • 細胞内局在

      Cytoplasmic

    • 参照データベース

      • 別名

        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      画像

      • Western blot - Anti-beta Galactosidase antibody (ab12081)
        Western blot - Anti-beta Galactosidase antibody (ab12081)
        Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg

        Secondary
        Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size: 116 kDa
        Observed band size: 120 kDa why is the actual band size different from the predicted?


        Exposure time: 90 seconds

      参考文献 (6)

      ab12081 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab12081 は 6 報の論文で使用されています。

      • Gong X  et al. Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib. Cancer Cell 32:761-776.e6 (2017). PubMed: 29232554
      • Brzoska E  et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). ICC . PubMed: 25890097
      • Mojiri A  et al. Hypoxia results in upregulation and de novo activation of von Willebrand factor expression in lung endothelial cells. Arterioscler Thromb Vasc Biol 33:1329-38 (2013). PubMed: 23580145
      • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). WB . PubMed: 23223527
      • Zimmer C  et al. Role of Fgf8 signalling in the specification of rostral Cajal-Retzius cells. Development 137:293-302 (2010). IHC-Fr ; Mouse . PubMed: 20040495
      • Liebner S  et al. Wnt/beta-catenin signaling controls development of the blood-brain barrier. J Cell Biol 183:409-17 (2008). IHC-Fr . PubMed: 18955553

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-beta Galactosidase antibody

       Excellent
      Abreviews
      Application
      Western blot
      Sample
      Escherichia coli Cell lysate - whole cell (bact beta-gal protein expressed in rat epithelial)
      Loading amount
      20 µg
      Specification
      bact beta-gal protein expressed in rat epithelial
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Dr. Silvia Finnemann

      Verified customer

      投稿 Aug 13 2012

      Question

      Inquiry: Hello. I just received a shipment of ab12081 Goat anti LacZ lot. As I was aliquoting the antibody for long term storage at -20 I only was able to retrieve 70uL of antibody but there should be 100ul in the vial. The vial was centrifuged before opening and the inside of the lid was checked for residual liquid.. none was found. Also the antibody solution had a noticeable cloudy white appearance that I have not noticed in other lots of this product before. Thanks in advance for your assistance. Cheers Steve

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not with the full amount as stated on the datasheet and for the inconvenience this has caused. I have issued a free of charge replacement for a full vial of ab12081 with the order number xxxxx. As to the cloudy appearance I would recommend spinning the antibody down for a few minutes and drawing the antibody from the top. You should not see any reduction in affinity. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Question

      My Fluorescent signal is weak using xylene based mounting media.

      Read More
      Answer

      Thank you for your phone call.

      As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with DAPI. I hope this heps, if not, please let me know and I will be happy to assist you further.

      Read More

      Question

      Thanks for your help on this matter. Much appreciated.  I have attached an email showing the purchase of this anti lacZ antibody from March 24 2011... just short of 6 months.  The current lot # we are using is 134764. Would you happen to know if there is a different lot to try?   Also I was hoping to just place the order through Cedarlane again if that is OK with you?  Thanks again

      Read More
      Answer

      Thank you for your email. Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane. Looking forward to your reply.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com