Anti-beta Catenin 抗体 [CTNNB1/2030R] (ab237984)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CTNNB1/2030R] to beta Catenin
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-beta Catenin antibody [CTNNB1/2030R]
beta Catenin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [CTNNB1/2030R] to beta Catenin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Protein Arraymore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human beta Catenin.
Database link: P35222 -
ポジティブ・コントロール
- WB: HeLa cell lysate. IHC-P: Human tonsil and cervical carcinoma tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
CTNNB1/2030R -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. |
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Protein Array |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. |
Protein Array
Use at an assay dependent concentration. |
ターゲット情報
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機能
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
組織特異性
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. -
関連疾患
Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
配列類似性
Belongs to the beta-catenin family.
Contains 12 ARM repeats. -
翻訳後修飾
Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. - Information by UniProt
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参照データベース
- Entrez Gene: 1499 Human
- Omim: 116806 Human
- SwissProt: P35222 Human
- Unigene: 476018 Human
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別名
- b-catenin antibody
- Beta catenin antibody
- Beta-catenin antibody
see all
画像
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Formalin-fixed, paraffin-embedded human cervical carcinoma tissue stained for beta Catenin using ab237984 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for beta Catenin using ab237984 at 2 μg/ml in immunohistochemical analysis.
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Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 85 kDa -
ab237984 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab237984 は 2 報の論文で使用されています。
- Tang M et al. Evaluation of B7-H3 Targeted Immunotherapy in a 3D Organoid Model of Craniopharyngioma. Biomolecules 12:N/A (2022). PubMed: 36551172
- Wu CE et al. Cinnamaldehyde enhances apoptotic effect of oxaliplatin and reverses epithelial-mesenchymal transition and stemnness in hypoxic colorectal cancer cells. Exp Cell Res 383:111500 (2019). PubMed: 31306656