Application
Western blot
Sample
Human Tissue lysate - whole (Lung tumor lysate)
Loading amount
5 µg
Specification
Lung tumor lysate
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/4000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rb IgG (H&L)-HRP, Cell Signalling #7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Femto
Exposure
10 second(s)
Bands
Specific: 95 kDa
Positive control
Ran 5, 10, or 20 microgram/lane of human lung tumor lysate.
Negative control
Secondary Ab only; no bands observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Mike Campa
Verified customer
投稿 Apr 04 2006