Anti-beta Catenin 抗体 (ab2365)
Key features and details
- Rabbit polyclonal to beta Catenin
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
製品の概要
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製品名
Anti-beta Catenin antibody
beta Catenin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to beta Catenin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Chicken, Human, Xenopus laevis -
免疫原
Synthetic peptide within Human beta Catenin aa 750 to the C-terminus. The exact sequence is proprietary.
Database link: P35222 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 85 kDa. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 10 minutes at room temperature. |
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| IP | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
組織特異性
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. -
関連疾患
Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
配列類似性
Belongs to the beta-catenin family.
Contains 12 ARM repeats. -
翻訳後修飾
Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. - Information by UniProt
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参照データベース
- Entrez Gene: 395964 Chicken
- Entrez Gene: 1499 Human
- Entrez Gene: 12387 Mouse
- Entrez Gene: 84353 Rat
- Omim: 116806 Human
- SwissProt: P35222 Human
- SwissProt: Q02248 Mouse
- SwissProt: Q9WU82 Rat
see all -
別名
- b-catenin antibody
- Beta catenin antibody
- Beta-catenin antibody
see all
画像
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Western blot - Anti-beta Catenin antibody (ab2365)Anti-beta Catenin antibody (ab2365) at 1/25 dilution + A431 cell lysate
Predicted band size: 85 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab2365)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for beta Catenin using ab2365 at 1/250 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-beta Catenin antibody (ab2365)Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to beta Catenin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 85kDa; beta Catenin.
プロトコール
参考文献 (37)
ab2365 は 37 報の論文で使用されています。
- Chung SY et al. Elucidation of the mechanism underlying CD44v6-induced transformation of IEC-6 normal intestinal epithelial cells. J Cell Physiol 235:194-209 (2020). PubMed: 31219187
- Altay G et al. Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues. Front Bioeng Biotechnol 8:294 (2020). PubMed: 32318564
- Nestor-Bergmann A et al. Decoupling the Roles of Cell Shape and Mechanical Stress in Orienting and Cueing Epithelial Mitosis. Cell Rep 26:2088-2100.e4 (2019). PubMed: 30784591
- Chu SY et al. Mechanical stretch induces hair regeneration through the alternative activation of macrophages. Nat Commun 10:1524 (2019). PubMed: 30944305
- Higashi T et al. Comprehensive analysis of formin localization in Xenopus epithelial cells. Mol Biol Cell 30:82-95 (2019). PubMed: 30379611
