Application
Western blot
Sample
Rat Tissue lysate - whole (Testis)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (7.5%)
Loading amount
30 µg
Specification
Testis
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Detection
Detection method
ecl
Exposure
30 second(s)
Bands
Specific: 94 kDa Non-specific: 80, 50 kDa
Additional data
Additional Notes
there is always 3 bands appearance whether we follow chemiluminescent or fluorescence detection.
Abcam response
This antibody is not validated for use on the sample type tested (rat). Based on this review there is no cross-reactivity with rat under these conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Zafar Khan
Verified customer
投稿 Apr 01 2022