beta-Amyloid Peptide (42-1) (human), Inactive control (ab120481)
Key features and details
- Inactive control for Amyloid beta (1-42) (ab120301) peptide
- CAS Number: 317366-82-8
- Soluble in 0.1% NH4OH to 1mg/ml
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
beta-Amyloid Peptide (42-1) (human), Inactive control -
製品の詳細
Inactive control for Amyloid beta (1-42) (ab120301) peptide -
CAS 番号
317366-82-8 -
構造式
製品の特性
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分子量
4514.08 -
分子式
C203H311N55O60S -
配列
AIVVGGVMLGIIAGKNSGVDEAFFVLKQHHVEYGSDHRFEAD -
PubChem 登録番号
71581486 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in 0.1% NH4OH to 1mg/ml -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Amyloid β (42-1) human peptide should be initially dissolved at a concentration of 1mg/ml in 100% HFIP (1,1,1,3,3,3-hexafluoro-2-propanol). This solution should be incubated at room temperature for 1 hour, with occasional vortexing at a moderate speed. Next, the solution should be sonicated for 10 minutes in a water bath sonicator. The HFIP/peptide solution should then be dried under a gentle stream of nitrogen gas. 100% DMSO should be used to re-suspend the peptide. This solution should be incubated at room temperature for 12 minutes, with occasional vortexing. The final solution should then be aliquoted into smaller volumes and stored at -80 °C. For a working solution, add 500-1000 µl of D-PBS (depending on the final concentration to be used) to the peptide stock solution and incubate for 2h at room temperature to allow for peptide aggregation.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Synthetic
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研究分野
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab120481 は 1 報の論文で使用されています。
- Kaisis E et al. Protofibrillar Amyloid Beta Modulation of Recombinant hCaV2.2 (N-Type) Voltage-Gated Channels. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 36558910