Anti-beta Actin 抗体 - Loading Control (ab8229)
Key features and details
- Goat polyclonal to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Fish, Monkey, Chinese hamster
- Isotype: IgG
このターゲットにつきましては、リコンビナント・ウサギ・モノクローナル抗体も販売しています
製品の概要
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製品名
Anti-beta Actin antibody - Loading Control
beta Actin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to beta Actin - Loading Control -
由来種
Goat -
特異性
This antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Fish, Monkey, Chinese hamster
交差が予測される動物種: Goat, a wide range of other species
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta Actin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available asab13772) -
ポジティブ･コントロール
- WB: HeLa, NIH3T3, PC12 and CHOK1 whole cell lysates.
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特記事項
This antibody has been designed for use as a loading control and is ideal for this purpose.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
This antibody has been designed for use as a loading control and is ideal for this purpose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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アプリケーション
Our Abpromise guarantee covers the use of ab8229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
see all -
別名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
画像
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Western blot - Anti-beta Actin antibody - Loading Control (ab8229)All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1/1000 dilution
Lane 1 : HeLa whole cell (Human)
Lane 2 : 3T3 cell (Mouse)
Lane 3 : Rabbit Liver
Lane 4 : MDCK cell (Dog)
Lane 5 : EBTr cell (Cow)
Lane 6 : SL-29 cell (Chicken)
Lane 7 : CHO cell (Chinese Hamster)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutesSecondary antibody - rabbit anti-goat HRP (ab6741)
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Western blot - Anti-beta Actin antibody - Loading Control (ab8229)Lanes 1-4 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 5% BSA.)
Lanes 5-8 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 3% Milk.)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lanes 3 & 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lanes 4 & 8 : CHO-K1 cell lysate Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin (lanes 1-4) or 3% milk (lanes 5-8) before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using an anti-Goat antibody conjugated to HRP, and visualised using ECL development solution. A non-specific band at 50-kDa is observed on western blots of some of our batches of ab8229. We have found that milk blocking is more effective than BSA blocking in eliminating this non-specific binding.
参考文献 (160)
ab8229 は 160 報の論文で使用されています。
- Geng S et al. Bioactive phospho-therapy with black phosphorus for in vivo tumor suppression. Theranostics 10:4720-4736 (2020). PubMed: 32308745
- Piccinin E et al. PGC-1a induced browning promotes involution and inhibits lactation in mammary glands. Cell Mol Life Sci 76:5011-5025 (2019). PubMed: 31154462
- Zhang Q et al. Green tea polyphenols attenuate LPS-induced inflammation through upregulating microRNA-9 in murine chondrogenic ATDC5 cells. J Cell Physiol 234:22604-22612 (2019). PubMed: 31102286
- Senfter D et al. High impact of miRNA-4521 on FOXM1 expression in medulloblastoma. Cell Death Dis 10:696 (2019). PubMed: 31541075
- He H et al. Iron Overload Damages the Endothelial Mitochondria via the ROS/ADMA/DDAHII/eNOS/NO Pathway. Oxid Med Cell Longev 2019:2340392 (2019). PubMed: 31781327
