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  1. Link

    beta-actin-antibody-loading-control-ab8229.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Anti-beta Actin 抗体 - Loading Control (ab8229)

  • Datasheet
  • SDS
Reviews (11)Q&A (1)References (199)

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Western blot - Anti-beta Actin antibody - Loading Control (ab8229)
  • Western blot - Anti-beta Actin antibody - Loading Control (ab8229)

Key features and details

  • Goat polyclonal to beta Actin - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Chinese hamster
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-beta Actin antibody [SP124] (ab115777)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-beta Actin antibody - Loading Control
    beta Actin 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to beta Actin - Loading Control
  • 由来種

    Goat
  • 特異性

    This antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Chinese hamster
    交差が予測される動物種: Goat, Fish, Monkey, a wide range of other species
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab13772)

  • ポジティブ・コントロール

    • WB: HeLa, NIH3T3, PC12 and CHOK1 whole cell lysates.
  • 特記事項

    This antibody has been designed for use as a loading control and is ideal for this purpose.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.025% Sodium azide
    Constituent: PBS

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    This antibody has been designed for use as a loading control and is ideal for this purpose.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Immunizing Peptide (Blocking)

    • Human beta Actin peptide (ab13772)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)
  • Related Products

    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
    • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8229の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (11)
1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772).
特記事項
WB
1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772).

ターゲット情報

  • 機能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 配列類似性

    Belongs to the actin family.
  • 翻訳後修飾

    ISGylated.
  • 細胞内局在

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Omim: 102630 Human
    • SwissProt: P60706 Chicken
    • SwissProt: P48975 Chinese hamster
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    see all
  • 別名

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

画像

  • Western blot - Anti-beta Actin antibody - Loading Control (ab8229)
    Western blot - Anti-beta Actin antibody - Loading Control (ab8229)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1/1000 dilution

    Lane 1 : HeLa whole cell (Human)
    Lane 2 : 3T3 cell (Mouse)
    Lane 3 : Rabbit Liver
    Lane 4 : MDCK cell (Dog)

    Lane 5 : EBTr cell (Cow)
    Lane 6 : SL-29 cell (Chicken)
    Lane 7 : CHO cell (Chinese Hamster)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 5 minutes


    Secondary antibody - rabbit anti-goat HRP (ab6741)

  • Western blot - Anti-beta Actin antibody - Loading Control (ab8229)
    Western blot - Anti-beta Actin antibody - Loading Control (ab8229)
    Lanes 1-4 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 5% BSA.)
    Lanes 5-8 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 3% Milk.)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lanes 2 & 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lanes 3 & 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lanes 4 & 8 : CHO-K1 cell lysate Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin (lanes 1-4) or 3% milk (lanes 5-8) before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using an anti-Goat antibody conjugated to HRP, and visualised using ECL development solution.  A non-specific band at 50-kDa is observed on western blots of some of our batches of ab8229. We have found that milk blocking is more effective than BSA blocking in eliminating this non-specific binding. 

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (199)

ab8229 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8229 は 199 報の論文で使用されています。

  • Chen ZF  et al. Oncogenic potential of macrophage-capping protein in clear cell renal cell carcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33236143
  • Frederiksen SB  et al. Identification of SRSF10 as a regulator of SMN2 ISS-N1. Hum Mutat 42:246-260 (2021). PubMed: 33300159
  • Brampton C  et al. ABCC6 deficiency promotes dyslipidemia and atherosclerosis. Sci Rep 11:3881 (2021). PubMed: 33594095
  • Hussein RM  et al. Chlorogenic acid, quercetin, coenzyme Q10 and silymarin modulate Keap1-Nrf2/heme oxygenase-1 signaling in thioacetamide-induced acute liver toxicity. Life Sci 277:119460 (2021). PubMed: 33811899
  • Qu Y  et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 12 Abreviews or Q&A

Western blot abreview for Anti-beta Actin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Dec 04 2017

Western blot abreview for Anti-beta Actin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Colon cancer cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Abcam user community

Verified customer

投稿 Jul 29 2015

Western blot abreview for Anti-beta Actin antibody - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 May 07 2014

Western blot abreview for Anti-beta Actin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
30 µg
Specification
neuronal
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Read More

DR. Nicola Bright

Verified customer

投稿 Nov 28 2012

Western blot abreview for Anti-beta Actin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human C4-2 cells)
Loading amount
20 µg
Specification
human C4-2 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Nov 17 2011

Western blot abreview for Anti-beta Actin antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (PBMC)
Loading amount
20 µg
Specification
PBMC
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Oct 05 2011

Western blot abreview for Anti-beta Actin antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain tissue)
Loading amount
25 µg
Specification
Brain tissue
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Oct 30 2009

Western blot abreview for Anti-beta Actin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Loading amount
5 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 18 2009

Western blot abreview for Anti-beta Actin antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Mixed Hippocampal Culture)
Loading amount
100 µg
Specification
Mixed Hippocampal Culture
Treatment
24hrs
Gel Running Conditions
Non-reduced Denaturing (10% Tris HCl)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Feb 01 2008

Question

Can you confirm: Does anti-beta Actin antibody (ab8229) react with swine myocardium homogenate?

Read More

Abcam community

Verified customer

Asked on Dec 05 2011

Answer

We have not tested ab8229 on swine samples, but based on the level of homology between the immunogen sequence and the swine sequence (96%), we expect ab8229 to bind to swine samples, although we cannot guarantee it.

Read More

Abcam Scientific Support

Answered on Dec 05 2011

1-10 of 12 Abreviews or Q&A

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