Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord dorsal horn)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
30 µg
Specification
spinal cord dorsal horn
Blocking step
1x Rapid block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA
Dilution
1/8000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 03 2016