Western blot abreview for Anti-beta Actin antibody [AC-15]

Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Tissue lysate - whole (CNS tissue)
Specification
CNS tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk+ PBS+0.1% Tween 20
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 42 kDa
Exposure
5 minute(s) and 0 second(s)

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投稿 Aug 01 2014

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