Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample
Mouse Tissue sections (Embryonic brain, 14 days, 16 micron)
Specification
Embryonic brain, 14 days, 16 micron
Permeabilization
Yes - Triton X-100
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0.1% Triton X-100, 1% serum
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 Donkey Anti-Mouse IgG (H+L) Antib
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/700
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 08 2014