Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: 3% BSA (TBS-tween)
Dilution
1/10000
Secondary antibody
Dilution
1/6000
Name
Non-Abcam antibody was used: Goat anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
3 second(s)
Bands
Specific: 42 kDa
Additional data
Additional Notes
Lysis buffer: Ripa buffer + Protease inhibitors (1:1000)
Anti-beta Actin antibody was used in conjunction with Anti-GAPDH antibody (36Kda).
Note that Anti-beta Actin antibody could not be re-used. Make fresh dilutions for each WB experiment.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 03 2016