リコンビナントRabMAb

Anti-BDNF 抗体 [EPR1292] - BSA and Azide free (ab271873)

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Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Flow Cytometry - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
  • Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1292] to BDNF - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-BDNF antibody [EPR1292] - BSA and Azide free
    BDNF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1292] to BDNF - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human brain tissue, human bladder cancer tissue; Coronal sections from fetal (SNc) and post-natal (VTA) WT and Rgs6-/- mice.; ICC/IF: HeLa cells; Flow Cyt: HeLa cells; IHC-Fr: Mouse and Rat cerebrum tissue.

  • 特記事項

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 - 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    ab271873 is the carrier-free version of ab108319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-Fr
Mouse
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
IHC-P
Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.See IHC antigen retrieval protocols.Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
IHC-P
Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.See IHC antigen retrieval protocols.Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

  • 組織特異性

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

  • 関連疾患

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome

  • 配列類似性

    Belongs to the NGF-beta family.

  • 翻訳後修飾

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

    Immunohistochemistry (Frozen sections) analysis of rat cerebral cortex tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified ab108319 at 1:500 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).
  • Flow Cytometry - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Flow Cytometry - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified ab108319 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).
  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using unpurified ab108319 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108319).
  • Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)
    Anti-BDNF antibody [EPR1292] - BSA and Azide free (ab271873)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271873 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271873 は論文での使用が確認できていません。

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