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Anti-BDNF 抗体 [35928.11] (ab10505)

Reviews (1)Q&A (3)References (13)

Key features and details

  • Mouse monoclonal [35928.11] to BDNF
  • Suitable for: ELISA, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-BDNF antibody [EPR21211] (ab213323)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-BDNF antibody [35928.11]
    BDNF 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [35928.11] to BDNF

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISA, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant human BDNF (rhBDNF) expressed in Sf 21 insect cells.

  • ポジティブ･コントロール

    • Brain tissue lysate

  • 特記事項

    Endotoxin: <10 ng/mg by LAL method.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab10505の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

  • 組織特異性

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

  • 関連疾患

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome

  • 配列類似性

    Belongs to the NGF-beta family.

  • 翻訳後修飾

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    参考文献 (13)

    ab10505 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab10505 は 13 報の論文で使用されています。

    • Sun Q  et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
    • Zhang L  et al. LncRNA Rik-203 Contributes to Sevoflurane Induced Neurotoxicity? Front Med (Lausanne) 7:353 (2020). PubMed: 32793611
    • Jasim H  et al. Daytime changes of salivary biomarkers involved in pain. J Oral Rehabil 47:843-850 (2020). PubMed: 32277715
    • Jasim H  et al. Saliva as a medium to detect and measure biomarkers related to pain. Sci Rep 8:3220 (2018). PubMed: 29459715
    • Yu J  et al. IL-1ß Stimulates Brain-Derived Neurotrophic Factor Production in Eutopic Endometriosis Stromal Cell Cultures: A Model for Cytokine Regulation of Neuroangiogenesis. Am J Pathol 188:2281-2292 (2018). PubMed: 30031725
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-BDNF antibody [35928.11]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - other (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (4-15% stain free gel)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 6°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 15 2016

    Question

    Hola de nuevo Abcam:
    Uno de nuestros clientes adquirió estos 3 anticuerpos con nosotros, ellos los alicuotaron y almacenaron a -20°C, ahora que tratan de usarlos ninguno de los tres funciona, nos podrán ayudar, aún están dentro de los 6 meses de abpromise?
    AB10419 LOTE XXXXXXXX
    AB69 LOTE XXXXXXXXXX
    AB10505 LOTE XXXXXXXXX
    Muchas Gracias.¡

    Read More
    Answer

    Gracias por contactarnos de nuevo.

    ¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

    ¿En qué aplicación y especies se están usando?

    Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

    Gracias por la colaboración. Quedo a la espera de tu respuesta.

    Read More

    Question

    Dear Sir/Madam, My question is whether this antibody can be used in order to detect rat bdnf protein. I am afraid we have ordered the wrong antibody. If your answer is yes, where should I expect a band? 14.3 kDa (mature BDNF) or 32 kDa (pro-BDNF)?

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear you have been experiencing problems with Anti-BDNF antibody ab10505 in rat samples.

    Unfortunately, this antibody has not been tested with rat samples and therefore we cannot guarantee that it works. Having said this, the sequence similarity of the human BDNF (immunogen) compared to the rat orthologue is 96% and therefore there is a possibility that this antibody could detect rat BDNF.

    If you wish, we could have a look at your protocol to work out if there is anything that can be done to improve the results. Therefore, I have attached our questionnaire which will help you put the information we require together very easily.

    To my knowledge, it would depend on the tissue type and any kind of treatment whether to expect the mature, plasmin-converted 14.3 kDa form or the 32 kDa pro-BDNF.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    here the response on your questionnaire. In the second testing, instead of one band at 60 kDa, a lot of bands showed up. Maybe you can identify the problem by looking on our protocol, otherwise I'm open for any suggestions. Thanks in advance, 1. Order details: • Batch number: 174226 • Abcam order or Purchase order number: do not know, order was placed at January, the 18th and antibody arrived at January, the 31th • Antibody storage conditions (temperature/reconstitution etc) Reconstitution: PBS; Storage at -20°C in aliquots 2. Please describe the problem (high background, wrong band size, more bands, no band etc). high background in two of three tests, wrong band size (60 kDa instead of 27,8 kDa) in the other (pictures in the attachment) 3. On what material are you testing the antibody in WB? • Species: human • Cell extract or Nuclear extract: cell extract • Purified protein or Recombinant protein: - 3. The lysate • How much protein was loaded: 50 µg • What lysis buffer was used: RIPA buffer • What protease inhibitors were used: Protease Inhibitor Cocktail (Sigma) • What loading buffer was used: NUPAGE LDS Sample Buffer (Invitrogen) • Did you heat the samples: temperature and time: 95°C for 5 minutes 4. Electrophoresis/Gel conditions/ Transfer conditions • Reducing or non reducing gel: Reducing gel • Gel percentage : 4%-12% • Transfer conditions: Tank Blotting on PVDF membrane 5. Blocking conditions • Buffer: TBS-Tween • Blocking agent: milk, BSA, serum, what percentage: milk, 10% • Incubation time: 1 h • Incubation temperature: room temperature 6. Primary Antibody • Specification (in which species was it raised against): mouse • At what dilution(s) have you tested this antibody: 1:300 • What dilution buffer was used: milk • Incubation time: over night (~18 h) • Incubation temperature: 4°C • What washing steps were done: 3x5’ with TBS-T, 2x5’ with TBS 7. Secondary Antibody • Specification (in which species was it raised against)? rabbit • At what dilution(s) have you tested this antibody: 1:10000 • Incubation time 1 h • Wash steps: 3x5’ with TBS-T, 2x5’ with TBS • Do you know whether the problems you are experiencing come from the secondary? I don’t think so. 8. Detection method ECl, ECl+, other detection method: ECL+ 9. Background bands • Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): Yes • Is the blocking step sufficient? Yes • Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) Yes • At what size are the bands migrating? Could they be degradation products of your target? No degradation product, the bands are slower migrating than the specific product • Please provide an image of your blot (as an e-mail attachment, a faxed image is not sufficient) provided as jpeg 10. Did you apply positive and negative controls along with the samples? Please specify. We applied protein from glioblastoma as positive controls 11. Optimization attempts • How many times have you tried the Western? 3 times • Do you obtain the same results every time e.g. are background bands always in the same place? Yes • What steps have you altered? We took other samples and increased the washing time other incubation with the secondary

    Read More
    Answer

    Thank you very much for providing your protocol details. I think the 60kDa band you originally observed is a result of the homodimerization of BDNF in the samples in your first experiment so this is very promising (for example the datasheet of another BDNF antibody shows banding patterns in rat brain, see the datasheet for ab46176) (https://www.abcam.com/BDNF-antibody-ab46176.html). I suspect that further denaturing and reducing of the samples will help the separation of the dimers. It would be good to include a positive control such as brain lysate/hippocampus lysate for example, as levels of BDNF may vary a lot in glioblastoma cells. I suspect that the non specific bands you observe in the next experiments are due to the blocking buffer. I would recommend to incubate the membrane in 5% milk (and to try 5% BSA which in our hands works better) for 1 hour only, and to incubate the antibody overnight at 4C in TBST (Tween 0.1%). If you still experience problems please do not hesitate to contact me,

    Read More

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