Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Rabbit cornea. Limbal epithelial area. Only progen)
Specification
Rabbit cornea. Limbal epithelial area. Only progen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking dilution
Dilution
1/40
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 488 Goat anti Mouse IgG2a
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Blocking solution: 5% BSA in PBS + 0,1% Triton-X-100
It seems to detect the appropriate cells in the correct area, but images are not completely conclusive.
Abcam response
This antibody has not yet been validated in rabbit samples by our lab. We invite any scientists who would like to test this antibody in an untested species or application to use our AbTrial for risk free testing. Contact our Scientific Support Team for more details.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Noelia Andollo
Verified customer
投稿 Jun 23 2015