Anti-BCRP/ABCG2 抗体 [BXP-21] (ab3380)
Key features and details
- Mouse monoclonal [BXP-21] to BCRP/ABCG2
- Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-BCRP/ABCG2 antibody [BXP-21]
BCRP/ABCG2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BXP-21] to BCRP/ABCG2 -
由来種
Mouse -
特異性
BXP-21 reacts with an internal epitope of BCRP/ABCG2, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.
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アプリケーション
適用あり: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, ICCmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within BCRP/ABCG2 aa 250-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells. Caco Cell lysate (see second reference cited).
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特記事項
For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308
We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Constituent: 0.7% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
一次抗体 備考
Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone. -
ポリ/モノ
モノクローナル -
クローン名
BXP-21 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3380の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| ICC/IF | (2) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 24136221
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| ICC |
Use at an assay dependent concentration.
Acetone fixed cells. |
| 特記事項 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 24136221 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC
Use at an assay dependent concentration. Acetone fixed cells. |
ターゲット情報
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機能
Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. -
組織特異性
Highly expressed in placenta. Low expression in small intestine, liver and colon. -
配列類似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
翻訳後修飾
Glycosylation-deficient ABCG2 is normally expressed and functional. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9429 Human
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- Unigene: 480218 Human
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別名
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (106)
ab3380 は 106 報の論文で使用されています。
- Bartos Z & Homolya L Identification of Specific Trafficking Defects of Naturally Occurring Variants of the Human ABCG2 Transporter. Front Cell Dev Biol 9:615729 (2021). PubMed: 33634118
- Wu CP et al. Overexpression of Human ABCB1 and ABCG2 Reduces the Susceptibility of Cancer Cells to the Histone Deacetylase 6-Specific Inhibitor Citarinostat. Int J Mol Sci 22:N/A (2021). PubMed: 33807514
- Zhang J et al. Rapamycin Antagonizes BCRP-Mediated Drug Resistance Through the PI3K/Akt/mTOR Signaling Pathway in mPRa-Positive Breast Cancer. Front Oncol 11:608570 (2021). PubMed: 33912444
- Versele R et al. Ketone Bodies Promote Amyloid-ß1-40 Clearance in a Human in Vitro Blood-Brain Barrier Model. Int J Mol Sci 21:N/A (2020). PubMed: 32023814
- Chen HD et al. Novel mutations in BRCA2 intron 11 and overexpression of COX-2 and BIRC3 mediate cellular resistance to PARP inhibitors. Am J Cancer Res 10:2813-2831 (2020). PubMed: 33042619