Anti-BCMA 抗体 - C-terminal (ab5972)

Submit a review Q&A (2)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
  • Western blot - Anti-BCMA antibody - C-terminal (ab5972)
  • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)

Key features and details

  • Rabbit polyclonal to BCMA - C-terminal
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-BCMA antibody - C-terminal
    BCMA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to BCMA - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human BCMA (C terminal).
    (Peptide available as ab6087)

  • ポジティブ･コントロール

    • Human spleen tissue lysate, K562 or U937 whole cell lysates.

  • 特記事項

    Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    BCMA Antibody is affinity chromatography purified via peptide column.

  • 一次抗体 備考

    Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa.

ターゲット情報

  • 機能

    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.

  • 組織特異性

    Expressed in mature B-cells, but not in T-cells or monocytes.

  • 関連疾患

    Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.

  • 配列類似性

    Contains 1 TNFR-Cys repeat.

  • 細胞内局在

    Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B cell maturation antigen antibody
      • B cell maturation factor antibody
      • B cell maturation protein antibody
      • B-cell maturation protein antibody
      • BCM antibody
      • BCMA antibody
      • CD269 antibody
      • CD269 antigen antibody
      • TNFRSF17 antibody
      • TNR17_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 17 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)

      ab5972 at 10µg/ml staining BCMA in human spleen cells by IHC

    • Western blot - Anti-BCMA antibody - C-terminal (ab5972)
      Western blot - Anti-BCMA antibody - C-terminal (ab5972)
      Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5 µg/ml. Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5 µg/ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)
      Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)
      Immunofluorescence of BCMA in Human Spleen cells using ab5972 at 10 ug/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)

      Immunohistochemistry of BCMA in human spleen tissue with BCMA antibody at 5 µg/ml.

    参考文献 (8)

    ab5972 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5972 は 8 報の論文で使用されています。

    • Carrillo-Ballesteros FJ  et al. B-cell activating factor receptor expression is associated with germinal center B-cell maintenance. Exp Ther Med 17:2053-2060 (2019). PubMed: 30783477
    • Howard L  et al. TWE-PRIL reverse signalling suppresses sympathetic axon growth and tissue innervation. Development 145:N/A (2018). PubMed: 30337376
    • Dou H  et al. APRIL promotes non-small cell lung cancer growth and metastasis by targeting ERK1/2 signaling. Oncotarget 8:109289-109300 (2017). PubMed: 29312608
    • McWilliams TG  et al. TNF superfamily member APRIL enhances midbrain dopaminergic axon growth and contributes to the nigrostriatal projection in vivo. Exp Neurol 298:97-103 (2017). ICC/IF ; Mouse . PubMed: 28911883
    • Dou H  et al. APRIL, BCMA and TACI proteins are abnormally expressed in non-small cell lung cancer. Oncol Lett 12:3351-3355 (2016). WB, IHC-P ; Human . PubMed: 27900003
    • Osório C  et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). IHC-Fr ; Mouse . PubMed: 24444792
    • Müller N  et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . PubMed: 24728308
    • Brady G  et al. Downregulation of RUNX1 by RUNX3 requires the RUNX3 VWRPY sequence and is essential for Epstein-Barr virus-driven B-cell proliferation. J Virol 83:6909-16 (2009). PubMed: 19403666

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Where does the antibody bind to BCMA: extracellular or intracellular domain?

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    Answer

    The immunogen is from the C-terminal region of BCMA, so the antibody binds the intracellular domain.

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    Question


    Inquiry: does this antibody react with mouse BCMA?

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    Answer

    Thank you for contacting us.
    This product has not been tested in mouse samples but it is unlikely to react, as the immunogen has very low homology with mouse.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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