Anti-BCMA 抗体 - C-terminal (ab5972)
Key features and details
- Rabbit polyclonal to BCMA - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-BCMA antibody - C-terminal
BCMA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BCMA - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human BCMA (C terminal).
(Peptide available asab6087) -
ポジティブ･コントロール
- Human spleen tissue lysate, K562 or U937 whole cell lysates.
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特記事項
Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
BCMA Antibody is affinity chromatography purified via peptide column. -
一次抗体 備考
Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5972の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa. |
ターゲット情報
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機能
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. -
組織特異性
Expressed in mature B-cells, but not in T-cells or monocytes. -
関連疾患
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2. -
配列類似性
Contains 1 TNFR-Cys repeat. -
細胞内局在
Cell membrane. Endomembrane system. Perinuclear Golgi-like structures. - Information by UniProt
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参照データベース
- Entrez Gene: 608 Human
- Omim: 109545 Human
- SwissProt: Q02223 Human
- Unigene: 2556 Human
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別名
- B cell maturation antigen antibody
- B cell maturation factor antibody
- B cell maturation protein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
ab5972 at 10µg/ml staining BCMA in human spleen cells by IHC
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Western blot - Anti-BCMA antibody - C-terminal (ab5972)Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5µ g/ml. Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)Immunofluorescence of BCMA in Human Spleen cells using ab5972 at 10 ug/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)Immunohistochemistry of BCMA in human spleen tissue with BCMA antibody at 5 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab5972 は 8 報の論文で使用されています。
- Carrillo-Ballesteros FJ et al. B-cell activating factor receptor expression is associated with germinal center B-cell maintenance. Exp Ther Med 17:2053-2060 (2019). PubMed: 30783477
- Howard L et al. TWE-PRIL reverse signalling suppresses sympathetic axon growth and tissue innervation. Development 145:N/A (2018). PubMed: 30337376
- Dou H et al. APRIL promotes non-small cell lung cancer growth and metastasis by targeting ERK1/2 signaling. Oncotarget 8:109289-109300 (2017). PubMed: 29312608
- McWilliams TG et al. TNF superfamily member APRIL enhances midbrain dopaminergic axon growth and contributes to the nigrostriatal projection in vivo. Exp Neurol 298:97-103 (2017). ICC/IF ; Mouse . PubMed: 28911883
- Dou H et al. APRIL, BCMA and TACI proteins are abnormally expressed in non-small cell lung cancer. Oncol Lett 12:3351-3355 (2016). WB, IHC-P ; Human . PubMed: 27900003
- Osório C et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). IHC-Fr ; Mouse . PubMed: 24444792
- Müller N et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . PubMed: 24728308
- Brady G et al. Downregulation of RUNX1 by RUNX3 requires the RUNX3 VWRPY sequence and is essential for Epstein-Barr virus-driven B-cell proliferation. J Virol 83:6909-16 (2009). PubMed: 19403666
