Anti-BCMA 抗体 - C-terminal (ab5972)

Submit a review Q&A (2)References (8)

Key features and details

  • Rabbit polyclonal to BCMA - C-terminal
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-BCMA antibody - C-terminal
    BCMA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to BCMA - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human BCMA (C terminal).
    (Peptide available as ab6087)

  • ポジティブ･コントロール

    • Human spleen tissue lysate, K562 or U937 whole cell lysates.

  • 特記事項

    Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    BCMA Antibody is affinity chromatography purified via peptide column.

  • 一次抗体 備考

    Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa.

ターゲット情報

  • 機能

    Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.

  • 組織特異性

    Expressed in mature B-cells, but not in T-cells or monocytes.

  • 関連疾患

    Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.

  • 配列類似性

    Contains 1 TNFR-Cys repeat.

  • 細胞内局在

    Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B cell maturation antigen antibody
      • B cell maturation factor antibody
      • B cell maturation protein antibody
      • B-cell maturation protein antibody
      • BCM antibody
      • BCMA antibody
      • CD269 antibody
      • CD269 antigen antibody
      • TNFRSF17 antibody
      • TNR17_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 17 antibody
      see all

    画像

    • Western blot - Anti-BCMA antibody - C-terminal (ab5972)
      Western blot - Anti-BCMA antibody - C-terminal (ab5972)
      Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5 µg/ml. Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5 µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)

      ab5972 at 10µg/ml staining BCMA in human spleen cells by IHC

    • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)
      Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)
      Immunofluorescence of BCMA in Human Spleen cells using ab5972 at 10 ug/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)

      Immunohistochemistry of BCMA in human spleen tissue with BCMA antibody at 5 µg/ml.

    参考文献 (8)

    ab5972 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5972 は 8 報の論文で使用されています。

    • Carrillo-Ballesteros FJ  et al. B-cell activating factor receptor expression is associated with germinal center B-cell maintenance. Exp Ther Med 17:2053-2060 (2019). PubMed: 30783477
    • Howard L  et al. TWE-PRIL reverse signalling suppresses sympathetic axon growth and tissue innervation. Development 145:N/A (2018). PubMed: 30337376
    • Dou H  et al. APRIL promotes non-small cell lung cancer growth and metastasis by targeting ERK1/2 signaling. Oncotarget 8:109289-109300 (2017). PubMed: 29312608
    • McWilliams TG  et al. TNF superfamily member APRIL enhances midbrain dopaminergic axon growth and contributes to the nigrostriatal projection in vivo. Exp Neurol 298:97-103 (2017). ICC/IF ; Mouse . PubMed: 28911883
    • Dou H  et al. APRIL, BCMA and TACI proteins are abnormally expressed in non-small cell lung cancer. Oncol Lett 12:3351-3355 (2016). WB, IHC-P ; Human . PubMed: 27900003
    • Osório C  et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). IHC-Fr ; Mouse . PubMed: 24444792
    • Müller N  et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . PubMed: 24728308
    • Brady G  et al. Downregulation of RUNX1 by RUNX3 requires the RUNX3 VWRPY sequence and is essential for Epstein-Barr virus-driven B-cell proliferation. J Virol 83:6909-16 (2009). PubMed: 19403666

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Where does the antibody bind to BCMA: extracellular or intracellular domain?

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    Answer

    The immunogen is from the C-terminal region of BCMA, so the antibody binds the intracellular domain.

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    Question


    Inquiry: does this antibody react with mouse BCMA?

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    Answer

    Thank you for contacting us.
    This product has not been tested in mouse samples but it is unlikely to react, as the immunogen has very low homology with mouse.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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