Anti-Bcl6 抗体 [GI191E] (ab241549)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [GI191E] to Bcl6
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Bcl6 antibody [GI191E]
Bcl6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GI191E] to Bcl6 -
由来種
Mouse -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human Bcl6 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P41182 -
ポジティブ・コントロール
- WB: Raji and Ramos whole cell lysate. Mouse brain, heart and kidney tissue lysate. IP: Ramos whole cell lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
GI191E -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab241549の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.403 µg/ml. Predicted molecular weight: 79 kDa.
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IP |
Use a concentration of 13.433 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.403 µg/ml. Predicted molecular weight: 79 kDa. |
IP
Use a concentration of 13.433 µg/ml. |
ターゲット情報
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機能
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
組織特異性
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
関連疾患
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
配列類似性
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
ドメイン
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
翻訳後修飾
Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 604 Human
- Entrez Gene: 12053 Mouse
- Omim: 109565 Human
- SwissProt: P41182 Human
- SwissProt: P41183 Mouse
- Unigene: 478588 Human
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別名
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
画像
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All lanes : Anti-Bcl6 antibody [GI191E] (ab241549) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 79 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Bcl6 antibody [GI191E] (ab241549) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 79 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1,3: 81 secs; Lane 2: 5 secs.
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Bcl6 was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab241549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab241549 at 1/500 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: Ramos whole cell lysate 10µg (Input).
Lane 2: ab241549 IP in Ramos whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab241549 in Ramos whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab241549 は 1 報の論文で使用されています。
- Feng D et al. Chromatin organizer SATB1 controls the cell identity of CD4+ CD8+ double-positive thymocytes by regulating the activity of super-enhancers. Nat Commun 13:5554 (2022). PubMed: 36138028