Anti-Bcl6 抗体 (ab272859)
Key features and details
- Rabbit polyclonal to Bcl6
- Suitable for: IHC-Fr, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bcl6 antibody
Bcl6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bcl6 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P HumanIP HumanWB MouseHuman
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免疫原
Recombinant fragment within Human Bcl6. The exact sequence is proprietary. Center region.
Database link: P41182 -
ポジティブ･コントロール
- WB: Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, Raji and C2C12 whole cell lysate. IHC-P: Human tonsil tissue. IHC-Fr: Mouse cerebellum tissue. IP: Raji whole cell lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-Fr |
Mouse
|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Mouse
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
1/100 - 1/1000.
|
|
| WB |
1/500 - 1/3000.
|
|
| IHC-P |
1/100 - 1/1000.
|
|
| IP |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
IHC-Fr
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. |
|
IP
1/100 - 1/500. |
ターゲット情報
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機能
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
組織特異性
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
関連疾患
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
配列類似性
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
ドメイン
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
翻訳後修飾
Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 604 Human
- Entrez Gene: 12053 Mouse
- Entrez Gene: 303836 Rat
- Omim: 109565 Human
- SwissProt: P41182 Human
- SwissProt: P41183 Mouse
- Unigene: 478588 Human
- Unigene: 347398 Mouse
see all -
別名
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-Bcl6 antibody (ab272859)
Frozen sections of mouse cerebellum tissue stained for Bcl6 using ab272859 at 1/250 dilution in immunohistochemical analysis. Counterstain: NF-H (red). Fluoroshield™ with DAPI (blue).
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Western blot - Anti-Bcl6 antibody (ab272859)All lanes : Anti-Bcl6 antibody (ab272859) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 (mouse glioblastoma cancer cell line) whole cell lysate
Lane 3 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody (ab272859)Paraffin embedded human tonsil tissue stained for Bcl6 using ab272859 at 10 μg/ml in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-Bcl6 antibody (ab272859)All lanes : Anti-Bcl6 antibody (ab272859) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Immunoprecipitation - Anti-Bcl6 antibody (ab272859)7.5% SDS-PAGE.
Immunoprecipitation of BCL6 from Raji (human Burkitt's lymphoma cell line) whole cell lysate by ab272859, 3 ug.
All lanes : Anti-Bcl6 antibody (ab272859) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Lane 2 : Control: pre-immune rabbit IgG at 3 µg
Lane 3 : Immunoprecipitation of BCL6 by ab272859 at 3 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab272859 は論文での使用が確認できていません。
