Anti-Bcl-XL 抗体 (ab2568)
Key features and details
- Rabbit polyclonal to Bcl-XL
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bcl-XL antibody
Bcl-XL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bcl-XL -
由来種
Rabbit -
特異性
This antibody detects the 30 kDa Bcl-XL protein. It does not react with other Bcl-2 family members. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
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ポジティブ･コントロール
- HeLa whole cell lysate (ab29545) can be used as a positive control in WB.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
組織特異性
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. -
翻訳後修飾
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. -
細胞内局在
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. - Information by UniProt
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参照データベース
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q64373 Mouse
- SwissProt: P53563 Rat
- Unigene: 516966 Human
see all -
別名
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
画像
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Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappaab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA (red) = Alexa Fluor®568-conjugated Wheat Germ Agglutinin
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献
ab2568 は 18 報の論文で使用されています。
- Miller IP et al. Beneficial Role of ROS in Cell Survival: Moderate Increases in H2O2 Production Induced by Hepatocyte Isolation Mediate Stress Adaptation and Enhanced Survival. Antioxidants (Basel) 8:N/A (2019). PubMed: 31581418
- Du L et al. Everolimus inhibits breast cancer cell growth through PI3K/AKT/mTOR signaling pathway. Mol Med Rep 17:7163-7169 (2018). PubMed: 29568883
- Farina AR et al. TrkAIII signals endoplasmic reticulum stress to the mitochondria in neuroblastoma cells, resulting in glycolytic metabolic adaptation. Oncotarget 9:8368-8390 (2018). PubMed: 29492201
- Sang Q et al. Curcumin Protects an SH-SY5Y Cell Model of Parkinson's Disease Against Toxic Injury by Regulating HSP90. Cell Physiol Biochem 51:681-691 (2018). PubMed: 30463061
- Chen L et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). PubMed: 29212196
