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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-Bcl-XL 抗体 (ab2568)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (18)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)
  • Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)

Key features and details

  • Rabbit polyclonal to Bcl-XL
  • Suitable for: ICC
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Bcl-XL protein (His tag) (ab180062)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

医薬用外毒物

製品の概要

  • 製品名

    Anti-Bcl-XL antibody
    Bcl-XL 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Bcl-XL
  • 由来種

    Rabbit
  • 特異性

    This antibody detects the 30 kDa Bcl-XL protein. It does not react with other Bcl-2 family members.
  • アプリケーション

    適用あり: ICCmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Bcl-XL. Synthetic peptide surrounding amino acid 56 of human Bcl-xL
    Database link: Q07817
    (Peptide available as ab52114)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    Affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Bcl-XL protein (His tag) (ab180062)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2568の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (1)
Use at an assay dependent concentration.
特記事項
ICC
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 組織特異性

    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 配列類似性

    Belongs to the Bcl-2 family.
  • ドメイン

    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻訳後修飾

    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 細胞内局在

    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Target information above from: UniProt accession Q07817 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 598 Human
    • Omim: 600039 Human
    • SwissProt: Q07817 Human
    • Unigene: 516966 Human
    • 別名

      • Apoptosis regulator Bcl X antibody
      • Apoptosis regulator Bcl-X antibody
      • Apoptosis regulator BclX antibody
      • B cell lymphoma 2 like antibody
      • B2CL1_HUMAN antibody
      • Bcl 2 like 1 protein antibody
      • Bcl X antibody
      • Bcl xL antibody
      • BCL XL/S antibody
      • Bcl xS antibody
      • Bcl-2-like protein 1 antibody
      • Bcl2 Like 1 antibody
      • Bcl2 related gene antibody
      • Bcl2-L-1 antibody
      • BCL2L antibody
      • Bcl2l1 antibody
      • BCLX antibody
      • BclXL antibody
      • BclXs antibody
      • DKFZp781P2092 antibody
      • PPP1R52 antibody
      • Protein phosphatase 1 regulatory subunit 52 antibody
      see all

    画像

    • Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)
      Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)
      ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)
      Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa

      ab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
      WGA (red) = Alexa Fluor®568-conjugated Wheat Germ Agglutinin

      See Abreview

    プロトコール

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (18)

    ab2568 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2568 は 18 報の論文で使用されています。

    • Miller IP  et al. Beneficial Role of ROS in Cell Survival: Moderate Increases in H2O2 Production Induced by Hepatocyte Isolation Mediate Stress Adaptation and Enhanced Survival. Antioxidants (Basel) 8:N/A (2019). PubMed: 31581418
    • Du L  et al. Everolimus inhibits breast cancer cell growth through PI3K/AKT/mTOR signaling pathway. Mol Med Rep 17:7163-7169 (2018). PubMed: 29568883
    • Farina AR  et al. TrkAIII signals endoplasmic reticulum stress to the mitochondria in neuroblastoma cells, resulting in glycolytic metabolic adaptation. Oncotarget 9:8368-8390 (2018). PubMed: 29492201
    • Sang Q  et al. Curcumin Protects an SH-SY5Y Cell Model of Parkinson's Disease Against Toxic Injury by Regulating HSP90. Cell Physiol Biochem 51:681-691 (2018). PubMed: 30463061
    • Chen L  et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). PubMed: 29212196
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-BCL2L1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (RAW 264.7)
    Specification
    RAW 264.7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 May 19 2012

    Western blot abreview for Anti-BCL2L1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Platelets)
    Loading amount
    20 µg
    Specification
    Platelets
    Treatment
    Calcium Ionophore for 1 h
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 Nov 14 2011

    Flow Cytometry abreview for Anti-BCL2L1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Platelets)
    Specification
    Platelets
    Preparation
    Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-X100 in 2% BSA for 15min
    Gating Strategy
    Platelets
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    投稿 Dec 22 2010

    Question

    DESCRIPTION OF THE PROBLEM Non-specific band The Bcl-xl antibody ab2586 recognises strongly (only) a band of 48 kDa on Western blots of total cell extracts of rat hepatocytes (in milk, TBS+0.1% Tween-20 or the same without the Tween). We used the recommended dilution of it. Any ideas why/ how to correct this? SAMPLE total cell extracts of rat hepatocytes PRIMARY ANTIBODY Ab2568 1 ug/ml DETECTION METHOD ECL ANTIBODY STORAGE CONDITIONS -20 C, thawed for the first time. SAMPLE PREPARATION homogenised on ice, boiled in laemmli AMOUNT OF PROTEIN LOADED 100 ug ELECTROPHORESIS/GEL CONDITIONS acrylamide/bis 12/0.5 TRANSFER AND BLOCKING CONDITIONS semi dry blotting, 5 % skimmed milk for 1 h, primary and secondary Ab in milk SECONDARY ANTIBODY anti rabbit-HRP BioRad, 1:5000 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? TBS pH 7.6 with or without 0.1 % Tween-20

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2007

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We have received some excellent feedback for this antibody in the form of customer "Abreviews". They all detail a strong band of approximately 30KDa. It is puzzling to me why this antibody detects a band of this size as the approach that you are using is one that I would recommend. To further optimise this antibody I would recommend that you perform a no-primary control experiment to determine whether this band is the result of the primary antibody. I would also like to recommend that you reduce the amount of protein that you have been using from 100ug to 20-30ug as a mass of this amount may be increasing the degree of non specificity. It may also be useful to try an alternative blocking agent such as BSA. Our lab team frequently find that a change to the blocking agent results in improved more specific results. Should you continue to have difficulties please get back in touch with me.

    Read More

    Abcam Scientific Support

    Answered on Mar 05 2007

    Western blot abreview for Anti-BCL2L1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela and 293T cells)
    Loading amount
    10 µg
    Specification
    Hela and 293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 14 2006

    Immunoprecipitation abreview for Anti-BCL2L1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Retinal Pigment Epithelial cell line)
    Total protein in input
    500 µg
    Specification
    Retinal Pigment Epithelial cell line
    Immuno-precipitation step
    Protein A
    Read More

    Dr. Ingram Iaccarino

    Verified customer

    投稿 Apr 27 2006

    Western blot abreview for Anti-BCL2L1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RPE, MDA MB231, HeLa)
    Loading amount
    20 µg
    Specification
    RPE, MDA MB231, HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. Ingram Iaccarino

    Verified customer

    投稿 Apr 13 2006

    Western blot abreview for Anti-BCL2L1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 30 2005

    Question

    I am looking for an anti-Bcl-XL antibody that works in immunoprecipitation, I was thinking about the product ab3193 or ab2568, since both should work in immunoprecipitation, do you have any reference of published papers where these products have been used in immunoprecipitation assays?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 01 2004

    Answer

    Thank you for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets, including references that we are currently aware of. If you have any more questions please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Apr 01 2004

    Question

    Is the immunogen the whole BclXL or a fragment of it? I need a polyclonal ab against the whole folded Bcl XL.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 23 2004

    Answer

    Ab2568 is against a peptide. However, ab2568 actually utilizes the whole protein as immunogen.

    Read More

    Abcam Scientific Support

    Answered on Jan 23 2004

    1-10 of 12 Abreviews or Q&A

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