Anti-Bcl-XL 抗体 (ab2568)
Key features and details
- Rabbit polyclonal to Bcl-XL
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bcl-XL antibody
Bcl-XL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bcl-XL -
由来種
Rabbit -
特異性
This antibody detects the 30 kDa Bcl-XL protein. It does not react with other Bcl-2 family members. -
アプリケーション
適用あり: ICCmore details -
種交差性
交差種: Human -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2568の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
組織特異性
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. -
翻訳後修飾
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. -
細胞内局在
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. - Information by UniProt
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参照データベース
- Entrez Gene: 598 Human
- Omim: 600039 Human
- SwissProt: Q07817 Human
- Unigene: 516966 Human
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別名
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
画像
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Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry - Anti-Bcl-XL antibody (ab2568)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappaab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA (red) = Alexa Fluor®568-conjugated Wheat Germ Agglutinin
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab2568 は 18 報の論文で使用されています。
- Miller IP et al. Beneficial Role of ROS in Cell Survival: Moderate Increases in H2O2 Production Induced by Hepatocyte Isolation Mediate Stress Adaptation and Enhanced Survival. Antioxidants (Basel) 8:N/A (2019). PubMed: 31581418
- Du L et al. Everolimus inhibits breast cancer cell growth through PI3K/AKT/mTOR signaling pathway. Mol Med Rep 17:7163-7169 (2018). PubMed: 29568883
- Farina AR et al. TrkAIII signals endoplasmic reticulum stress to the mitochondria in neuroblastoma cells, resulting in glycolytic metabolic adaptation. Oncotarget 9:8368-8390 (2018). PubMed: 29492201
- Sang Q et al. Curcumin Protects an SH-SY5Y Cell Model of Parkinson's Disease Against Toxic Injury by Regulating HSP90. Cell Physiol Biochem 51:681-691 (2018). PubMed: 30463061
- Chen L et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). PubMed: 29212196