Anti-BCKDHA (phospho S293) 抗体 (ab200577)
Key features and details
- Rabbit polyclonal to BCKDHA (phospho S293)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-BCKDHA (phospho S293) antibody
BCKDHA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BCKDHA (phospho S293) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within BCKDHA (phospho S293). The exact sequence is proprietary.
Database link: P12694 -
ポジティブ・コントロール
- WB: NIH 3T3, CT26, CH27, TCMK-1, BW5147.3, Hep-G2, HeLa, and LNCaP Whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab200577の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/10000 - 1/25000. Predicted molecular weight: 50 kDa.
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特記事項 |
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WB
1/10000 - 1/25000. Predicted molecular weight: 50 kDa. |
ターゲット情報
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機能
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). -
関連疾患
Maple syrup urine disease 1A -
配列類似性
Belongs to the BCKDHA family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 468889 Chimpanzee
- Entrez Gene: 282149 Cow
- Entrez Gene: 484488 Dog
- Entrez Gene: 101128832 Gorilla
- Entrez Gene: 593 Human
- Entrez Gene: 12039 Mouse
- Entrez Gene: 100462553 Orangutan
- Entrez Gene: 100142666 Pig
see all -
別名
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
see all
画像
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All lanes : Anti-BCKDHA (phospho S293) antibody (ab200577) at 1/25000 dilution
Lane 1 : Hep-G2 Whole cell lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : LNCaP Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
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All lanes : Anti-BCKDHA (phospho S293) antibody (ab200577) at 1/25000 dilution
Lane 1 : NIH 3T3 Whole cell lysate
Lane 2 : CT26 Whole cell lysate
Lane 3 : CH27 Whole cell lysate
Lane 4 : TCMK-1 Whole cell lysate
Lane 5 : BW5147.3 Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab200577 は 14 報の論文で使用されています。
- Wu G et al. Exercise Enhances Branched-Chain Amino Acid Catabolism and Decreases Cardiac Vulnerability to Myocardial Ischemic Injury. Cells 11:N/A (2022). PubMed: 35626742
- Pontoizeau C et al. Neonatal gene therapy achieves sustained disease rescue of maple syrup urine disease in mice. Nat Commun 13:3278 (2022). PubMed: 35672312
- Lee J et al. BCAA Supplementation in Mice with Diet-induced Obesity Alters the Metabolome Without Impairing Glucose Homeostasis. Endocrinology 162:N/A (2021). PubMed: 33765118
- Yoshida N et al. Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity. iScience 24:103342 (2021). PubMed: 34805797
- Kaya E et al. Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease. J Clin Med 9:N/A (2020). PubMed: 32276303