保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
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精製度Immunogen affinity purified
特記事項（精製）Purified by peptide affinity chromatography method.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab90691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with BCKDHA peptide (ab134676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
機能The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
関連疾患Maple syrup urine disease 1A
配列類似性Belongs to the BCKDHA family.
- Information by UniProt
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling BCKDHA with ab90691 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I and II cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - BCKDHA. Right - Merge.
Anti-BCKDHA antibody (ab90691) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Gel concentration: 12%
This product has been referenced in:
- Singh R et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). Read more (PubMed: 29618935) »
- Kitsy A et al. Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes. PLoS One 9:e102615 (2014). WB ; Mouse . Read more (PubMed: 25050624) »