Anti-BCA1 抗体 (ab272874)
Key features and details
- Rabbit polyclonal to BCA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-BCA1 antibody
BCA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BCA1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC Recombinant fragmentICC/IF HumanIHC-P HumanWB Human
-
免疫原
Synthetic peptide within Human BCA1 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O43927 -
ポジティブ･コントロール
- IHC-P: Human esophageal carcinoma tissue. ICC/IF: HepG2 cells. WB: Transfected 293T whole cell extracts.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC |
Recombinant fragment
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. -
組織特異性
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 10563 Human
- Omim: 605149 Human
- SwissProt: O43927 Human
- Unigene: 100431 Human
-
別名
- ANGIE antibody
- ANGIE2 antibody
- B cell attracting chemokine 1 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody (ab272874)
Paraffin-embedded human esophageal carcinoma tissue stained for BCA1 using ab272874 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
-
Immunocytochemistry/ Immunofluorescence - Anti-BCA1 antibody (ab272874)4% PFA-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for BCA1 using ab272874 at 1/500 dilution in ICC/IF (green).
Blue: Hoechst 33342 staining.
-
Western blot - Anti-BCA1 antibody (ab272874)All lanes : Anti-BCA1 antibody (ab272874) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected (+) HEK-293T whole cell lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody15% SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody (ab272874)Paraffin-embedded human esophageal carcinoma tissue stained for BCA1 using ab272874 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272874 は論文での使用が確認できていません。
