BCA Protein Assay Kit (ab102536)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Assay time: 1 hr 30 min
- Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
- Sensitivity: 0.5 µg
製品の概要
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製品名
BCA Protein Assay Kit
BCA Protein キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Serum, Plasma, Cell culture extracts, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
> 0.5 µg -
検出範囲
0.5 µg - 30 µg -
全工程の試験時間
1h 30m -
製品の概要
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).
Support on how to determine protein concentration can be found here.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1000 tests BCA Reagent 1 x 100ml BSA Standard III 1 x 1ml Copper Reagent 1 x 2ml -
研究分野
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関連性
The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration. -
別名
- BCA
- Bicinchoninic acid
- Bicinchoninic acid protein
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (136)
ab102536 は 136 報の論文で使用されています。
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