BCA Protein Assay Kit (ab102536)

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I have attached a graph of absorbance values measured at various wavelengths of light for various concentrations of BSA. The data demonstrates that the maximum absorbance is at 562nm but there is little loss of absorbance on either side of this peak, +/- 5nm, so your 560nm filter will be fine for this assay. In any case, it is likely that what you have is a band-pass filter centered on 560nm but allowing light above and below this wavelength to pass through, within a defined range that includes 562nm.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

It all depends on the concentration of EDTA and Citrate. For instance, EDTA<10mM and sodium citrate <200mM can be used with a standard BCA assay. Please be aware that the BCA assay is based on Copper ion chemistry, therefore it is not advised to use any metal chelator (such as EDTA at higher concentration of the one suggested).

I can confirm that both BCA and Bradford protein quantification kits can be used for human serum and plasma samples. The main difference between the two kits is that the BCA assay uses a Copper ion based chemistry whereas the Bradford assay uses a Coomassie-dye based reaction to produce color. The addition of heparin on your sample can form heparin-copper complex that can chelate out the copper ions in the BCA reagent. Therefore, in your particular case I will suggest to use the Bradford Assay (ab102535).

Thank you for contacting us.

If you are interested in knowing the compatibility of diffrent substances then following link will be helpful. They use the same compound for estimation as we use;

http://genome.yale.edu/images/a/aa/BCA.pdf


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Merci de nous avoir contactés.
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Par conséquent, je suis ravie de vous envoyer un certificat de conformité pour BCA Protein Quantification Kit, ab102536 (voir pièce ci-jointe).
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J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.
Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Vielen Dank für Ihre Anfrage.

Ich kann bestätigen, dass die meisten Methoden zur Proteinbestimmung nicht funktionieren wenn Detergenzien enthalten sind.

Die beste und robusteste Methode ist deshalb der BCA Protein Assay. Diese Methode ist kompatibel mit bis zu 5% Triton-X und 10mM EDTA.

Ich kann außerdem empfehlen, eine Positivkontrolle mit BSA im selben Puffer mitlaufen zu lassen.

Wir bieten den BCA Protein Assay in Form des folgenden Kits an:

ab102536

https://www.abcam.com/BCA-Protein-Assay-Kit-ab102536.html (or use the following: https://www.abcam.com/BCA-Protein-Assay-Kit-ab102536.html).

Ich hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg mit Ihren Versuchen.

Thank you for your email and sorry for my late reply.

I think if an expert in the field has suggested this method has worked for his group it may well be worth trying. What you could always do is try both methods of lysis and probe the samples to see which method works best in your hands.

The quantification for the protein should not be a problem with the BCA kit. The reason why detergent can interfere with the assay is because higher concentrations of detergents tend to bind to proteins tightly and interfere with the final colour development. However, BCA based assays are very detergents tolerant. If you want to make sure that accurate readings are being taken, you could always dilute your samples lysed in the sample buffer by at least 2 in PBS or similar then take the reading. This will make the total detergent content below 5% and should therefore not have a significant affect on the assay.

I hope this information has been of help. If I can be of any further assistance, please do let me know.

Thank you for your reply.

I will then let the order through as it stands. We will endeavor to have the product with you as soon as possible but as I am sure you can understand we are reliant on delivery companies to move the package on our behalf. I will keep you updated with its progress.

As for the BCA kit and the extra BSA you have received compared to the 1 mL described. This is not unusual, we tend to supply a larger volume of all our products than we advertise to take into account any pipetting performed on our side. We want to make sure that the customer never receives less than expected and therefore typically over fill, with up to 10% of product. This should have no effect on the concentration of BSA supplied.

We do advise aliquotting the Anti-Heme Oxygenase 1 antibody when stored at -20°C regardless of the fact that it is in 50% glycerol. This is to avoid the antibody going through multiple freeze/thaw cycles (or more accurately with the glycerol added, temperature changes). This can affect the activity of the antibody and as a precautionary measure this is what we would advise. It also helps in that it can reduce the chance of contamination of the stock.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

Thank you for your reply.

I have now placed your order for you. This is on the order number 1xxxxand xxxx. The DTT reducer is currently not directly in stock and it will therefore take a few weeks to reach you. I have therefore split the orders. The anti-HO1 antibody and BCA kit(ab13243 and ab102536 respectively) will be with you tomorrow.

The total cost of the order is €757 (you are only being charged one shipping charge of €22 in this total).

We do not currently sell timers but I have included one of our promotional ones in your order for free.

I also have a little further information to share in regards to the DTT reducer. I have been advised that this reagent is likely to be stable for up to 3 months if stored as directed on the datasheet (4°C). It may therefore be better to order it as you need it rather than stock up.

I can also confirm that the 10x10 cm Optiblot gels will be compatible with the Dual Cool Electrophoresis system (DCX-700) you are using. The best kit for you may therefore be the Optiblot Reducing Electrophoresis Kit (10x10) at 12% or 4-20% (kit https://www.abcam.com/ab119218or https://www.abcam.com/ab119220respectively).

I hope this information has been of help.Please do let me know if I can be of any further assistance.

Thank you for your reply.

The protocol provided with the BCA kit (ab102536) describes how to use the kit with a microplate reader. I have attached this document for your information. It is possible the kit could be adapted to be used with a nanodrop reader but some optimisation may be required on your part as we have not performed this ourselves.

I hope this information has been of help. Let me know if I can be of any further assistance.