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Anti-Bax 抗体 [5B7] (ab3191)

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Western blot - Anti-Bax antibody [5B7] (ab3191)
  • Flow Cytometry - Anti-Bax antibody [5B7] (ab3191)
  • Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)
  • Anti-Bax antibody [5B7] (ab3191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [5B7] to Bax
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-Bax antibody [5B7]
    Bax 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5B7] to Bax

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, Flow Cyt, IPmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • ポジティブ･コントロール

    • WB: A20 and C2C12 lysate. Flow Cyt: RAW 264.7 cells.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa.
Flow Cyt Use a concentration of 1.043 µg/ml.
IP Use a concentration of 13.9 µg/ml.

ターゲット情報

  • 機能

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

  • 組織特異性

    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

  • 配列類似性

    Belongs to the Bcl-2 family.

  • ドメイン

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • 細胞内局在

    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    画像

    • Western blot - Anti-Bax antibody [5B7] (ab3191)
      Western blot - Anti-Bax antibody [5B7] (ab3191)
      Lane 1 : Anti-Bax antibody [5B7] (ab3191) at 0.417 mg/ml
      Lane 2 : Anti-Bax antibody [5B7] (ab3191) at 0.417 µg/ml

      Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
      Lane 2 : C2C12 (Mouse myoblasts) lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 21 kDa


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry - Anti-Bax antibody [5B7] (ab3191)
      Flow Cytometry - Anti-Bax antibody [5B7] (ab3191)

      Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at a 1.043µg/ml  (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

    • Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)
      Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)

      Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml. Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
      Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
      Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
      Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Anti-Bax antibody [5B7] (ab3191)
      Anti-Bax antibody [5B7] (ab3191)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab3191 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3191 は論文での使用が確認できていません。

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