製品の概要

  • 製品名
    Anti-BAM22P antibody
  • 製品の詳細
    Rabbit polyclonal to BAM22P
  • 由来種
    Rabbit
  • 特異性
    This antibody recognizes mammalian BAM 22P.
  • アプリケーション
    適用あり: IHC-Fr, RIAmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mammals
  • 免疫原

    Synthetic peptide: synthetic BAM 22P.

  • 特記事項
    The antibody is routinely tested by dot blot.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
RIA 1/400000.

ターゲット情報

  • 関連性
    Bovine Adrenal Medulla peptide (BAM 22P). This opioid peptide, a 22 amino acid fragment of the enkephalin precursor preproenkephalin A, binds with high affinity to all three known opioid receptors (micro, kappa, and delta), indicating a possible function in pain transmission. Recent studies have implicated BAM 22P in sensory neuron activation.
  • 細胞内局在
    Cell Membrane
  • 参照データベース
  • 別名
    • PENK antibody
    • Proenkephalin A antibody

プロトコール

参考文献

ab18788 has not yet been referenced specifically in any publications.

レビューと Q&A

Answer

Thank you for your enquiry. This antibody is sourced externally. From correspondence with our originator I understand that you may have contacted them directly. However, I can tell you in response to your questions; 1 How has the antibody to BAM22P been characterized? A) It was previously characterized through radioimmunoassays but is now characterized through use of dot blots. 2 What is the selectivity of anti-BAM22P for the COOH terminus? A. There are 3 amino acids of importance as antigenic determinants, which are Pro-11, Glu-12, and the C-terminal Gly-22. I would also like to direct you to reference #2, listed below, as a source of helpful information regarding this topic. 3 Have you determined if this antibody shows cross-reactivity to the enkephalins, dynorphins or beta-endorphin? A) This antibody does not cross-react with Met-Enkephalin or BAM12P. 4 In your specification sheet, it was written that The antibody is routinely tested by dot blot. What does dot blot mean? What is this test for? A) A dot blot is a form of immunoblotting, where a small amount of antigen is loaded onto a piece of membrane in the form of a dot and the antibody is then added, followed by a secondary antibody conjugated to HRP. Developing is performed similar to that of a western blot where there is a colorimetric change, giving a positive or negative result. 5 Do you know any published literature in which your product of anti-BAM22P was used? If you do, please provide the information. A) Here are the references, which are also listed on our web-site, which discusses uses with this particular antibody. 1. Lembo PM, Grazzini E, Groblewski T, O'Donnell D, Roy MO, Zhang J, Hoffert C, Cao J, Schmidt R, Pelletier M, Labarre M, Gosselin M, Fortin Y, Banville D, Shen SH, Strom P, Payza K, Dray A, Walker P, Ahmad S (2002) Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRs. Nat Neurosci 5(3):201-209. 2. Baird A, Klepper R, Ling N (1984) In vitro and in vivo evidence that the C-terminus of preproenkephalin-A circulates as an 8500-dalton molecule. Proc Soc Exp Biol Med 175(3):304-308. 3. Bloch B, Baird A, Ling N, Benoit R, Guillemin R (1983) Immunohistochemical evidence that brain enkephalins arise from a precursor similar to adrenal preproenkephalin. Brain Res 263:251-257. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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