製品の概要

  • 製品名
    Anti-BAI1 associated protein 2 isoform 3 antibody
  • 製品の詳細
    Goat polyclonal to BAI1 associated protein 2 isoform 3
  • 由来種
    Goat
  • 特異性
    This antibody will only recognise isoform 3 of the three reported isoforms.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide: SMSSADVEVARF, corresponding to C terminal amino acids 509-520 of Human BAI1 associated protein 2 isoform 3.

  • ポジティブ・コントロール
    • HeLa cell lysates
  • 特記事項


    Brain-specific angiogenesis inhibitor (BAI1)-binding protein may be involved in neuronal growth-cone guidance.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考
    Brain-specific angiogenesis inhibitor (BAI1)-binding protein may be involved in neuronal growth-cone guidance.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human BAI1 associated protein 2 isoform 3 peptide (ab19041).

ターゲット情報

  • 関連性
    BAIAP2, a target of p53, has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This interaction at the cytoplasmic membrane is crucial to the function of this protein, which may be involved in neuronal growth-cone guidance. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis.
  • 細胞内局在
    Cell Membrane and Cytoplasmic
  • 参照データベース
  • 別名
    • BAI associated protein 2 antibody
    • BAIAP2 antibody
    • BAP2 antibody
    • Insulin receptor substrate p53 antibody
    • Insulin receptor substrate p53/p58 antibody
    • Insulin receptor substrate protein of 53 kDa antibody
    • IRSp53 antibody
    • Protein BAP2 antibody
    see all

画像

  • ab791 staining (1µg/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
  • Lane 1 : Anti-BAI1 associated protein 2 isoform 3 antibody (ab791) at 1 µg/ml
    Lane 2 : anti- DYKDDDDK Tag at 1/3000 dilution
    Lane 3 : Anti-BAI1 associated protein 2 isoform 3 antibody (ab791) at 1 mg/ml

    Lanes 1-2 : HEK293 lysate in RIPA buffer over expressing Human BAIAP2 with DYKDDDDK tag at 10 µg
    Lane 3 : Mock-transfected HEK293

プロトコール

参考文献

This product has been referenced in:
  • Asmar F  et al. Diffuse large B-cell lymphoma with combined TP53 mutation and MIR34A methylation: Another "double hit" lymphoma with very poor outcome? Oncotarget 5:1912-25 (2014). ChIP . Read more (PubMed: 24722400) »
  • Uslu K  et al. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice. J Immunol 192:3582-95 (2014). Read more (PubMed: 24646744) »
See all 2 Publications for this product

レビューと Q&A

Answer

Thank you for your enquiry. AB791 is "Goat polyclonal to BAI1 associated protein 2 isoform 3" and as we understand from your e-mail you are using Goat anti-Rabbit secondary. There seems to be some problem with the compatibility of the primary and the secondary antibody. We would suggest using either Donkey or Rabbit polyclonal to Goat IgG.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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