Anti-BAFF-R 抗体 (ab5965)
Key features and details
- Rabbit polyclonal to BAFF-R
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-BAFF-R antibody
BAFF-R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BAFF-R -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human BAFF-R (C terminal).
(Peptide available asab6086) -
ポジティブ・コントロール
- WB: Human, Mouse or Rat spleen tissue lysate. IHC: human lymphoid tissue (see review), human tonsil tissue
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
BAFF-R Antibody is affinity chromatography purified via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 19 kDa. | |
IHC-P | 1/1000 - 1/2000. |
ターゲット情報
-
機能
B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. -
組織特異性
Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes. -
関連疾患
Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4) [MIM:613494]; also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
配列類似性
Contains 1 TNFR-Cys repeat. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 115650 Human
- Entrez Gene: 72049 Mouse
- Entrez Gene: 500910 Rat
- Omim: 606269 Human
- SwissProt: Q96RJ3 Human
- SwissProt: Q9D8D0 Mouse
- Unigene: 344088 Human
- Unigene: 240047 Mouse
-
別名
- B cell activating factor receptor antibody
- B-cell-activating factor receptor antibody
- BAFF R antibody
see all
画像
-
Western blot analysis of BAFF-R in human (H), mouse (M), and rat (R) spleen tissue lysates with ab5965 at 5 µg/ml.
Western blot analysis of BAFF-R in human (H), mouse (M), and rat (R) spleen tissue lysates with ab5965 at 5 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (ab5965)
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human spleen tissue labeling BAFF-R with Anti-BAFF-R antibody (ab5965) at 20μg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (ab5965)
Immunohistochemistry of BAFF-R in human spleen tissue with BAFF-R antibody at 5 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (ab5965)
Immunohistochemistry (formalin-fixed paraffin-embedded) of BAFF-R in human tonsil tissue with ab5965 at 5 ug/ml.
プロトコール
データシートおよび資料
参考文献 (8)
ab5965 は 8 報の論文で使用されています。
- Cao Y et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
- Zhao Y et al. Factors associated with refractory autoimmune necrotizing myopathy with anti-signal recognition particle antibodies. Orphanet J Rare Dis 15:181 (2020). PubMed: 32641139
- Ma S et al. microRNA-96 promotes osteoblast differentiation and bone formation in ankylosing spondylitis mice through activating the Wnt signaling pathway by binding to SOST. J Cell Biochem 120:15429-15442 (2019). PubMed: 31111563
- Ramani B et al. Comparison of spinocerebellar ataxia type 3 mouse models identifies early gain-of-function, cell-autonomous transcriptional changes in oligodendrocytes. Hum Mol Genet 26:3362-3374 (2017). PubMed: 28854700
- Price JV et al. Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus. J Clin Invest 123:5135-45 (2013). PubMed: 24270423
- Birnbaum T et al. Expression of B-cell activating factor, a proliferating inducing ligand and its receptors in primary central nervous system lymphoma. Neurol Int 5:e4 (2013). PubMed: 23717783
- Hildebrand JM et al. A BAFF-R mutation associated with non-Hodgkin lymphoma alters TRAF recruitment and reveals new insights into BAFF-R signaling. J Exp Med 207:2569-79 (2010). WB ; Human . PubMed: 21041452
- Paterson JC et al. The differential expression of LCK and BAFF-receptor and their role in apoptosis in human lymphomas. Haematologica 91:772-80 (2006). WB, IHC-P ; Human . PubMed: 16769579