Anti-Baf180 抗体 [BL-39-2C3] - BSA free (ab272073)
![Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)](https://www.abcam.co.jp/ps/products/272/ab272073/Images/ab272073-1-ab243876334294antibaf180antibodybl392c3immunocytochemistry.jpg "Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-39-2C3] to Baf180 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
製品の概要
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製品名
Anti-Baf180 antibody [BL-39-2C3] - BSA free
Baf180 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BL-39-2C3] to Baf180 - BSA free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP Human
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免疫原
Recombinant fragment corresponding to Human Baf180 aa 1-293.
Database link: Q86U86 -
特記事項
This product is sold under License from Bethyl Laboratories, Inc.
ab272073 is the carrier-free version of ab243876. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Concentration information loading... -
特記事項（精製）
Recombinant antibody was purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BL-39-2C3 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC |
Human
|
| IHC-P |
Human
|
| IP |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation. -
組織特異性
Widely expressed. -
関連疾患
Defects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
配列類似性
Contains 2 BAH domains.
Contains 6 bromo domains.
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55193 Human
- Omim: 606083 Human
- SwissProt: Q86U86 Human
- Unigene: 189920 Human
- Unigene: 476320 Human
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別名
- BAF180 antibody
- BRG1-associated factor 180 antibody
- CG11375 antibody
see all
画像
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Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Baf180 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)](https://www.abcam.co.jp/ps/products/272/ab272073/Images/ab272073-2-ab243876334293antibaf180antibodybl392c3immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)Formalin-fixed, paraffin-embedded human Breast tissue stained for Baf180 using ab243876 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
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![Immunoprecipitation - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)](https://www.abcam.co.jp/ps/products/272/ab272073/Images/ab272073-3-ab243876334292antibaf180antibodybl392c3immunoprecipitation.jpg)
Immunoprecipitation - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)Baf180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution.
Lane 1: ab243876 IP in HEK-293T whole cell lysate.
Lane 2: Contol IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
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![Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)](https://www.abcam.co.jp/ps/products/272/ab272073/Images/ab272073-4-benefits-of-recombinant-antibodies.png)
Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272073 は論文での使用が確認できていません。