Anti-Bad (phospho S112) 抗体 [EPR1891(2)] - BSA and Azide free (ab248343)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free
Bad 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, IP, WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248343 is the carrier-free version of ab129192.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 4.70 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1891(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248343の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
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特記事項 |
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Dot blot
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa. |
ターゲット情報
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機能
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. -
組織特異性
Expressed in a wide variety of tissues. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻訳後修飾
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. -
細胞内局在
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 572 Human
- Entrez Gene: 12015 Mouse
- Entrez Gene: 64639 Rat
- Omim: 603167 Human
- SwissProt: Q92934 Human
- SwissProt: Q61337 Mouse
- SwissProt: O35147 Rat
- Unigene: 370254 Human
see all -
別名
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
see all
画像
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All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)
Lane 1 : Untreated C6 cells
Lane 2 : C6 cells treated with Calcyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?This data was developed using ab129192, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab129192, the same antibody clone in a different buffer formulation.ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
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This data was developed using ab129192, the same antibody clone in a different buffer formulation.Dot blot analysis of Bad (phospho S112) using ab129192 primary antibody at a dilution of 1/1000. ab97051 was used as a secondary antibody at a dilution of 1/100,000. Lane 1: Bad (pS112) phospho peptide
Lane 2: Bad non-phospho peptide Blocking and diluting buffer: 5% NFDM/TBST -
All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)
Lane 1 : Untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Calcyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?This data was developed using ab129192, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDaThis data was developed using ab129192, the same antibody clone in a different buffer formulation.
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This data was developed using ab129192, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248343 は論文での使用が確認できていません。