Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Bad (phospho S112) antibody [EPR1891(2)] - BSA and Azide free
Bad 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1891(2)] to Bad (phospho S112) - BSA and Azide free
アプリケーション適用あり: Dot blot, IP, WBmore details
適用なし: ICC/IF or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Bad. The exact sequence is proprietary.
Ab248343 is the carrier-free version of ab129192. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248343 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 4.70 x 10 -11 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab248343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.Can be blocked with Bad peptide (ab171725).|
機能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
組織特異性Expressed in a wide variety of tissues.
配列類似性Belongs to the Bcl-2 family.
ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
翻訳後修飾Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
細胞内局在Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
- Information by UniProt
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.