Anti-Bad (phospho S112) 抗体 (ab1435)

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Key features and details

  • Rabbit polyclonal to Bad (phospho S112)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Bad (phospho S112) antibody
    Bad 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Bad (phospho S112)

  • 由来種

    Rabbit

  • 特異性

    The antibody detects Bad only when phosphorylated at Ser 112.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能

    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

  • 組織特異性

    Expressed in a wide variety of tissues.

  • 配列類似性

    Belongs to the Bcl-2 family.

  • ドメイン

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • 翻訳後修飾

    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

  • 細胞内局在

    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

画像

  • Western blot - Anti-Bad (phospho S112) antibody (ab1435)
    Western blot - Anti-Bad (phospho S112) antibody (ab1435)
    Lanes 2-5 : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml

    All lanes : COS (African green monkey kidney fibroblast-like cell line) whole cell lysate

    Performed under reducing conditions.

    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    Lane 2: No TPA treatment. Lane 3: 5 minute TPA treatment. Lane 4: 30 minute TPA treatment. Lane 5: 60 minute TPA treatment.

    Anti-BAD antibody used as a loading control

参考文献 (0)

ab1435 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1435 は論文での使用が確認できていません。

レビューと Q&A

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Question

Customer is having problems getting this antibody to work in IP. He is using human cells and at the recommended concentration of 1 - 2 µg/ml. Batch # is 20426. Does the supplier have any protocol specifics for this antibody in IP? What type of cells was it tested with?

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Answer

The supplier of this antibody has informed me that ab1435 should be used at a concentration of 10 ug/ml for IP. I apologize for the error on the datasheet and have corrected it. I recommend trying ab1435 at 10 ug/ml and please let me know how this works out for you.

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