Anti-Bad (phospho S112) 抗体 (ab1435)

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Western blot - Anti-Bad (phospho S112) antibody (ab1435)

    Key features and details

    • Rabbit polyclonal to Bad (phospho S112)
    • Suitable for: WB
    • Reacts with: African green monkey
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Bad (phospho S112) antibody
      Bad 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Bad (phospho S112)

    • 由来種

      Rabbit

    • 特異性

      The antibody detects Bad only when phosphorylated at Ser 112.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: African green monkey

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1435の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 1 - 2 µg/ml.
    特記事項
    WB
    Use a concentration of 1 - 2 µg/ml.

    ターゲット情報

    • 機能

      Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

    • 組織特異性

      Expressed in a wide variety of tissues.

    • 配列類似性

      Belongs to the Bcl-2 family.

    • ドメイン

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

    • 翻訳後修飾

      Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

    • 細胞内局在

      Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
    • Information by UniProt

    • 別名

      • AI325008 antibody
      • BAD antibody
      • BAD_HUMAN antibody
      • BBC 2 antibody
      • BBC2 antibody
      • BBC6 antibody
      • Bcl 2 Antagonist of Cell Death antibody
      • Bcl 2 Binding Component 6 antibody
      • BCL X / BCL 2 Binding Protein antibody
      • BCL X Binding Protein antibody
      • Bcl XL/Bcl 2 Associated Death Promoter antibody
      • Bcl-2-binding component 6 antibody
      • Bcl-2-like protein 8 antibody
      • Bcl-XL/Bcl-2-associated death promoter antibody
      • Bcl2 antagonist of cell death antibody
      • BCL2 antagonist of cell death protein antibody
      • BCL2 associated agonist of cell death antibody
      • Bcl2 Associated Death Promoter antibody
      • BCL2 binding component 6 antibody
      • BCL2 binding protein antibody
      • Bcl2 Like 8 Protein antibody
      • Bcl2-L-8 antibody
      • BCL2L8 antibody
      • Proapoptotic BH3 Only Protein antibody
      see all

    画像

    • Western blot - Anti-Bad (phospho S112) antibody (ab1435)
      Western blot - Anti-Bad (phospho S112) antibody (ab1435)
      Lanes 2-5 : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml

      All lanes : COS (African green monkey kidney fibroblast-like cell line) whole cell lysate

      Performed under reducing conditions.

      Observed band size: 24 kDa why is the actual band size different from the predicted?



      Lane 2: No TPA treatment. Lane 3: 5 minute TPA treatment. Lane 4: 30 minute TPA treatment. Lane 5: 60 minute TPA treatment.

      Anti-BAD antibody used as a loading control

    参考文献 (0)

    ab1435 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1435 は論文での使用が確認できていません。

    レビューと Q&A

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    Question

    Customer is having problems getting this antibody to work in IP. He is using human cells and at the recommended concentration of 1 - 2 µg/ml. Batch # is 20426. Does the supplier have any protocol specifics for this antibody in IP? What type of cells was it tested with?

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    Answer

    The supplier of this antibody has informed me that ab1435 should be used at a concentration of 10 ug/ml for IP. I apologize for the error on the datasheet and have corrected it. I recommend trying ab1435 at 10 ug/ml and please let me know how this works out for you.

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