Anti-Bad (phospho S112) 抗体 (ab1435)
Key features and details
- Rabbit polyclonal to Bad (phospho S112)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-Bad (phospho S112) antibody
Bad 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bad (phospho S112) -
由来種
Rabbit -
特異性
The antibody detects Bad only when phosphorylated at Ser 112. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: African green monkey -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading...
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ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1435の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. -
組織特異性
Expressed in a wide variety of tissues. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻訳後修飾
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. -
細胞内局在
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. - Information by UniProt
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別名
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
see all
画像
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Lanes 2-5 : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml
All lanes : COS (African green monkey kidney fibroblast-like cell line) whole cell lysate
Performed under reducing conditions.
Observed band size: 24 kDa why is the actual band size different from the predicted?Lane 2: No TPA treatment. Lane 3: 5 minute TPA treatment. Lane 4: 30 minute TPA treatment. Lane 5: 60 minute TPA treatment.
Anti-BAD antibody used as a loading control
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab1435 は論文での使用が確認できていません。