製品の概要
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製品名Anti-Bad (phospho S112) antibody
Bad 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Bad (phospho S112)
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由来種Rabbit
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特異性The antibody detects Bad only when phosphorylated at Ser 112.
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アプリケーション適用あり: IP, WBmore details
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種交差性交差種: Mouse, Rat, Human
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
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Concentration information loading... -
ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab1435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
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組織特異性Expressed in a wide variety of tissues.
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配列類似性Belongs to the Bcl-2 family.
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ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
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翻訳後修飾Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
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細胞内局在Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 572 Human
- Entrez Gene: 12015 Mouse
- Entrez Gene: 64639 Rat
- Omim: 603167 Human
- SwissProt: Q92934 Human
- SwissProt: Q61337 Mouse
- SwissProt: O35147 Rat
- Unigene: 370254 Human
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別名
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
see all
画像
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Western blot - Anti-Bad (phospho S112) antibody (ab1435)All lanes : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml
All lanes : COS cell lysate. Anti-BAD antibody used as a loading control.
Performed under reducing conditions.
Observed band size: 24 kDa (why is the actual band size different from the predicted?)
Lane 2: No TPA treatment. Lane 3: 5 minute TPA treatment. Lane 4: 30 minute TPA treatment. Lane 5: 60 minute TPA treatment.
参考文献
ab1435 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"