Anti-Bad (phospho S112) 抗体 (ab1435)

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Western blot - Anti-Bad (phospho S112) antibody (ab1435)

    Key features and details

    • Rabbit polyclonal to Bad (phospho S112)
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Bad (phospho S112) antibody
      Bad 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Bad (phospho S112)

    • 由来種

      Rabbit

    • 特異性

      The antibody detects Bad only when phosphorylated at Ser 112.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab1435 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 1 - 2 µg/ml.

    ターゲット情報

    • 機能

      Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

    • 組織特異性

      Expressed in a wide variety of tissues.

    • 配列類似性

      Belongs to the Bcl-2 family.

    • ドメイン

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

    • 翻訳後修飾

      Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

    • 細胞内局在

      Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AI325008 antibody
      • BAD antibody
      • BAD_HUMAN antibody
      • BBC 2 antibody
      • BBC2 antibody
      • BBC6 antibody
      • Bcl 2 Antagonist of Cell Death antibody
      • Bcl 2 Binding Component 6 antibody
      • BCL X / BCL 2 Binding Protein antibody
      • BCL X Binding Protein antibody
      • Bcl XL/Bcl 2 Associated Death Promoter antibody
      • Bcl-2-binding component 6 antibody
      • Bcl-2-like protein 8 antibody
      • Bcl-XL/Bcl-2-associated death promoter antibody
      • Bcl2 antagonist of cell death antibody
      • BCL2 antagonist of cell death protein antibody
      • BCL2 associated agonist of cell death antibody
      • Bcl2 Associated Death Promoter antibody
      • BCL2 binding component 6 antibody
      • BCL2 binding protein antibody
      • Bcl2 Like 8 Protein antibody
      • Bcl2-L-8 antibody
      • BCL2L8 antibody
      • Proapoptotic BH3 Only Protein antibody
      see all

    画像

    • Western blot - Anti-Bad (phospho S112) antibody (ab1435)
      Western blot - Anti-Bad (phospho S112) antibody (ab1435)
      Lanes 2-5 : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml

      All lanes : COS (African green monkey kidney fibroblast-like cell line) whole cell lysate

      Performed under reducing conditions.

      Observed band size: 24 kDa
      why is the actual band size different from the predicted?



      Lane 2: No TPA treatment. Lane 3: 5 minute TPA treatment. Lane 4: 30 minute TPA treatment. Lane 5: 60 minute TPA treatment.

      Anti-BAD antibody used as a loading control

    参考文献 (0)

    ab1435 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1435 は論文での使用が確認できていません。

    レビューと Q&A

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    Question

    Customer is having problems getting this antibody to work in IP. He is using human cells and at the recommended concentration of 1 - 2 µg/ml. Batch # is 20426. Does the supplier have any protocol specifics for this antibody in IP? What type of cells was it tested with?

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    Answer

    The supplier of this antibody has informed me that ab1435 should be used at a concentration of 10 ug/ml for IP. I apologize for the error on the datasheet and have corrected it. I recommend trying ab1435 at 10 ug/ml and please let me know how this works out for you.

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