Key features and details
- Rabbit polyclonal to bacterial Alkaline Phosphatase (Biotin)
- Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
製品名Anti-bacterial Alkaline Phosphatase antibody (Biotin)
製品の詳細Rabbit polyclonal to bacterial Alkaline Phosphatase (Biotin)
アプリケーション適用あり: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISAmore details
種交差性交差種: Species independent
Full length protein (E. coli).
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
特記事項（精製）This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Our Abpromise guarantee covers the use of ab7321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA (1:4,000 to 1:20,000)
as well as other peroxidase-antibody based enzymatic assays.
関連性Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody