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  1. Link

    bacillus-cereus-antibody-ab20556.pdf

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Microbiology Organism Bacteria Gram positive bacteria Bacillus
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Anti-Bacillus cereus 抗体 (ab20556)

  • Datasheet
  • SDS
Reviews (2)Q&A (7)References (2)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

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出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Rabbit polyclonal to Bacillus cereus
  • Suitable for: ICC/IF
  • Isotype: IgG

こちらの製品もご検討ください

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関連製品

製品の概要

  • 製品名

    Anti-Bacillus cereus antibody
  • 製品の詳細

    Rabbit polyclonal to Bacillus cereus
  • 由来種

    Rabbit
  • 特異性

    Reacts with spores and vegetative cells of Bacillus cereus and Bacillus subtilis. Antiserum is unabsorbed and may cross-react with other Bacillus species.
  • アプリケーション

    適用あり: ICC/IFmore details
  • 種交差性

    交差種: Other species
  • 免疫原

    Tissue, cells or virus corresponding to Bacillus cereus. Purified spores of Bacillus cereus (ATCC 11778) and Bacillus subtilis (ATCC 9372).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Bacillus

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20556の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
Use at an assay dependent dilution.
特記事項
ICC/IF
Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    Bacillus cereus is a Gram-positive, facultatively aerobic sporeformer whose cells are large rods and whose spores do not swell the sporangium. These and other characteristics, including biochemical features, are used to differentiate and confirm the presence B. cereus, although these characteristics are shared with B. cereus var. mycoides, B. thuringiensis and B. anthracis. Differentiation of these organisms depends upon determination of motility (most B. cereus are motile), presence of toxin crystals (B. thuringiensis), hemolytic activity (B. cereus and others are beta hemolytic whereas B. anthracis is usually nonhemolytic), and rhizoid growth which is characteristic of B. cereus var. mycoides.
  • 細胞内局在

    Bacterial spore
  • 別名

    • B. cereus antibody
    • B.cereus antibody

プロトコール

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (2)

ab20556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab20556 は 2 報の論文で使用されています。

  • Gluschko A  et al. The ß2 Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes. Cell Host Microbe 23:324-337.e5 (2018). PubMed: 29544096
  • Wei S  et al. Dynabeads protein G antibody conjugates combined with modified brain heart infusion broth for the enrichment and separation of Bacillus cereus in artificially contaminated vegetables. Food Sci Biotechnol 25:941-947 (2016). PubMed: 30263358

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-9 of 9 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Bacillus cereus antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Bacteria Cell (Endospore)
Permeabilization
No
Specification
Endospore
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Glutaraldehyde
Read More

MR. Alan Cookson

Verified customer

投稿 Jul 14 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bacillus cereus antibody

Inconclusive
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (porcine plasma-based in vitro grown biofilm)
Specification
porcine plasma-based in vitro grown biofilm
Fixative
Paraformaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Nov 08 2012

Question

Could you please advance my Anti-Pseudomonas antibody (ab68538) abreview (no. 32931) to your scientific team?

I have a problem submitting it again due to „no secondary species“.

Thanks in advance for your cooperation.

Best regards

Read More

Abcam community

Verified customer

Asked on Nov 13 2012

Answer

Thank you for your enquiry.

This is to confirm that the status of this Abreview has been changed from edited to submitted. It will be processed shortly by a member of our Scientific Team.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Nov 13 2012

Question

Thank you for your response.

Regarding one yet unsubmited abreview (for Anti-Pseudomonas antibody (ab68538): I’m awaiting one more test result tomorrow which will close up the rating...

Regarding the discount codes I obtained from “AbTrial program” I would like to ask you a question: Are the codes active directly after submitting the abreview? Since my codes expire on Monday, 12/11/2012 is thus the last day I can order new antibodies... Am I right?

Thank you for response in advance.

Best regards

Read More

Abcam community

Verified customer

Asked on Nov 09 2012

Answer

Thank you for your response.

This is to confirm that the two other Abreviews have been published and the Discount codes are active. As soon as the third Abreview is submitted, its Discount code will be activated as well.

Good luck with the experiments and I am looking forward to receiving the Abreview.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Nov 09 2012

Question

I would like to submit abreviews for below mentioned antibodies, but I have an problem with the abreview form:

Our laboratory uses the EnVision® + Kit HRP. Rabbit/Mouse (DAB+) as a secondary antibody in the IHC protocol, which is actually a marked polymer and is not in the list of secondary antibodies. Abreview form reports an error and wants to have this field filled for successful abreview.

How should I proceed?

I look forward to hearing from you.

Best regards

Read More

Abcam community

Verified customer

Asked on Nov 07 2012

Answer

Thank you for your enquiry.

I have changed the status from edited to submitted of these two Abreviews (ab19980 and ab20556). They have come through to our internal system without any problem so they will be processed very shortly by one of the members of our Scientific Team.

However, it seems that the other Abreview ab68538 does not have the “Rating” information and the system recognizes it as a potential problem. I have resent this to you and ask you please look through all the information and resubmit it.

Thank you for your co-operation and I look forward to receiving your Abreviews.

Read More

Abcam Scientific Support

Answered on Nov 07 2012

Question

Thanks a lot for your responce.

I agree with Terms and Conditions after reading it again at https://www.abcam.com/collaborationdiscount. I hope, that my testing will produce at least satisfactory results.

Today I asked our Lab-manager to order antibodies, so we can start testing it as soon as possible after delivery.

I would like to ask you for sending me the below mentioned 3 discount codes which will be used after hopefully succesfull development of the staining procedure and submitting the Abreviews at abcam’s web.

Thank you again for your interest and time spent during our correspondence.

I look foreward to hearing from you.

Best regards,

Read More

Abcam community

Verified customer

Asked on Jul 12 2012

Answer

DISCOUNT CODES:

ab19980 - 100%ABR-E76ZR
ab20556 - 100%ABR-E76ZL
ab68538 - 100%ABR-E7657




Expiration date: 12/11/2012

I am very pleased to hear you would like to accept our offer and testab19980, ab20556 and ab68538in IHC-P.

This code will give you: 1 per discount code free PRIMARY ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Jul 12 2012

Question

I would like to test Anti-Enterococcus antibody (ab19980) in IHC-P application on biofilm and minipig tissue samples. But in the same experiment with same samples, I plan to detect Bacillus bacteria with Anti-Bacillus cereus antibody (ab20556) and Pseudomonas bacteria with Anti-Pseudomonas antibody (ab68538) also which are both not tested in IHC-P application yet. (And the fourth antibody to be ordered is Anti-Staphylococcus aureus antibody [704] (ab 37644) which is already IHC-P aprooved.)

It is much more simple and less time consuming to test all three mentionned antibodies in the same run in order to develop and implement the optimal staining protocol for our laboratory, but what if I order the antibodies separatelly what is profitable for me if discount will be asigned with each order even over paying the shipping 3 times?

I’ll be greatfull, If I could get a discount for all Abs what you probably understand.

It depends on what is more valuable for your company, whether money or application information.

Thanks for an answer in advance.



Best regards

Jan






















Jan Kučera, MSc.
Histologist
Laboratory for wound healing









Mobile: +420605969 129
Phone: +420465519 571
E-mail:jan.kucera@contipro.com









Contipro Pharma a.s.
561 02 Dolní Dobrouč, Czech Republic
http://www.contipro.com


































From: technical@abcam.com [mailto:technical@abcam.com]
Sent: Wednesday, July 04, 2012 3:05 PM
To: Kučera Jan
Subject: Reply from Abcam to your enquiry regarding ab69232, ab107092 [CCE3921219]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Jan

Thank you for your interest.

Normally, we can offer one discount code for testing at a time for one product only not for multiple products. Please do let me know which product you wish to test first and confirm the product code number and the application/species.

If you need any further assistance in the future, please do not hesitate to contact me.






Best regards,
Tanya

Tanya Bagrij, PhD
Scientific Support Supervisor
Abcam plc
www.abcam.com


Your original inquiry to Abcam:



Hallo Tanya,

Thank you for your response and information you’ve sent me.

I would like to continue in our previous correspondence regarding testing the antibodies in yet untested application.

After reading the terms and conditions at the web site you have mailed me, I would like to ask you some questions to understand the terms correctly.



Terms and conditions:

1. Limited number of discounts available, Abcam reserves the right to select candidates for this offer

2. Code must be redeemed within four months after issue date

3. Code is valid after submission of the Abreview to Abcam. All required fields of the Abreview must be completed

4. The discount will be valid for one order

5. This offer cannot be used in conjunction with other promotions. Credit notes for previous purchases can be redeemed against purchases in this promotion

6. Limit one discount code per primary antibody and protein purchased

7. Offer applies to primary antibodies and proteins

8. The discount code must be redeemed for the same product type as originally purchased e.g. primary antibody for primary antibody, protein for protein



Regarding article 1: I would like to order 3 yet untested primary antibodies (ab68538, ab19980 and ab20556) and one allready tested (ab37644). Will I get 3 discount codes?

Regarding article 4: Meaning one future order of any primary antibody in the same number as the number of discount code/s acquired?



If the assigning of discount codes depends on procedures resp. protocols I would like to test, please, do not hesitate to contact me and I will e-mail them by return.



Best regard







Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3921219]













Discover more at abcam.com

Read More

Abcam community

Verified customer

Asked on Jul 10 2012

Answer

Thank you for your response and your interest.

It is very unusual to offer a discount code for more than 1 product particularly when the application is not one of the standard immunoassays (Western blot, ELISA, Flow cytometry, Immunohistochemistry etc).

The technique you wish to use/test our products is not the classical IHC-P (formalin-fixed paraffin embedded immunohistochemistry) procedure. At this stage it is not know how frequently other customers will use these products in such an assay and how easy to modify the technique.

Could you please confirm how the tissue sections will be processed and fixed and whether you have any publication on this specific technique? We need to consider carefully the applicability of this assay.

I look forward to hearing from you soon.

Read More

Abcam Scientific Support

Answered on Jul 10 2012

Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 192715 DESCRIPTION OF THE PROBLEM The datasheet for this antibody indicates that vegetative cells and spores of both Bacillus cereus and Bacillus subtilis react with this antibody. What is the specific protein target of this antibody? Is this protein present on the external surface of both vegetative cells and spores? SAMPLE n/a PRIMARY ANTIBODY n/a DETECTION METHOD n/a POSITIVE AND NEGATIVE CONTROLS USED n/a SAMPLE PREPARATION n/a TRANSFER AND BLOCKING CONDITIONS n/a SECONDARY ANTIBODY n/a HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? n/a WHAT STEPS HAVE YOU ALTERED? n/a ADDITIONAL NOTES n/a

Read More

Abcam community

Verified customer

Asked on Dec 05 2006

Answer

Dear Ms. Kennett, Thank you for your enquiry. This is a polyclonal antibody and therefore it is recognizing multiple epitopes on multiple proteins. The exact proteins recognized have not been identified. When tested for immunofluorescence, reactivity with surface proteins is visible. Unfortunately, due to the small size of the cells and spores, it is difficult to see if intracellular staining may also occur. I hope that this information helps. Please don't hesitate to contact us for advice in the future.

Read More

Abcam Scientific Support

Answered on Dec 06 2006

Question

Is there any data available that shows that this product reacts with spores and vegetative cells of Bacillus cereus and Bacillus subtilis?

Read More

Abcam community

Verified customer

Asked on Nov 03 2005

Answer

Thank you for your enquiry. In answer to your question rab20556 is reactive with both spores and vegetative cells of Bacillus cereus and Bacillus subtilis. This testing was done in IFA. I hope this information helps.

Read More

Abcam Scientific Support

Answered on Nov 04 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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