製品の概要
-
製品名Anti-BACE1 antibody
BACE1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to BACE1
-
由来種Rabbit
-
アプリケーション適用あり: ICC, WB, ICC/IF, IHC-Pmore details
-
種交差性交差種: Mouse, Rat, Human, Drosophila melanogaster
-
免疫原
Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).
-
ポジティブ･コントロール
- IHC-P: Mouse brain tissue WB: Human brain tissue lysate, 3T3 lysate. ICC/IF: 3T3 cells
-
特記事項
Beta-site APP Cleaving Enzyme.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
-
バッファーPreservative: 0.02% Sodium azide
-
Concentration information loading... -
精製度Ion Exchange Chromatography
-
特記事項（精製）BACE Antibody is Ion exchange chromatography purified.
-
一次抗体 備考Beta-site APP Cleaving Enzyme.
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab2077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa.Can be blocked with BACE1 peptide (ab7883). We recommend overnight incubation at 4C and using 5% skim milk to block. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
ターゲット情報
-
機能Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
-
組織特異性Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
-
配列類似性Belongs to the peptidase A1 family.
-
ドメインThe transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
-
翻訳後修飾Glycosylated.
-
細胞内局在Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
- Information by UniProt
-
参照データベース
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all -
別名
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
画像
-
Western blot - Anti-BACE1 antibody (ab2077)All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml
Lane 1 : Human brain tissue
lysate with absence of blocking peptide
Lane 2 : Human brain tissue
lysate withBACE1 peptide (ab7883)
Lane 3 : Mouse
3T3 cell lysate
Observed band size: 70 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)Immunocytochemical analysis of NIH/3T3 cells, labeling BACE1 with ab2077 (20µg/ml).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody (ab2077)Immunohistochemistry of BACE in mouse brain tissue with BACE antibody at 2.5 µg/ml.
-
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)Image courtesy of an anonymous Abreview.ab2077 staining BACE1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI. -
Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)Image courtesy of an anonymous Abreview.ab2077 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.
参考文献
This product has been referenced in:
- Tang Y et al. Estrogen-related receptor alpha is involved in Alzheimer's disease-like pathology. Exp Neurol 305:89-96 (2018). Read more (PubMed: 29641978) »
- Hsu WH et al. The neuronal protection of a zinc-binding protein isolated from oyster. Food Chem Toxicol 114:61-68 (2018). Read more (PubMed: 29432843) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"