リコンビナントRabMAb

Anti-B7H4 抗体 [EPR23665-20] - BSA and Azide free (ab272707)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Immunoprecipitation - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Flow Cytometry - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
  • Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23665-20] to B7H4 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WB
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free
    B7H4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23665-20] to B7H4 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: SK-BR-3, MDA-MB-468, NIH:OVCAR-3 lysates. IHC-P: Human breast tissue, Human ovarian carcinoma and Human breast carcinoma tissues. Mouse breast carcinoma tissue. Mouse breast tissue. ICC/IF: 293T+OE-612 cells Flow Cyt: SK-BR-3 cells. IP: SK-BR-3 cell lysate.

  • 特記事項

    ab272707 is the carrier-free version of ab252438. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
IP
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.

ターゲット情報

  • 機能

    Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

  • 組織特異性

    Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.

  • 配列類似性

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Cell membrane. Expressed at the cell surface. A soluble form has also been detected.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B7 family member, H4 antibody
      • B7 H4 antibody
      • B7 homolog 4 antibody
      • B7 superfamily member 1 antibody
      • B7 superfamily, member 1 antibody
      • B7-H4 antibody
      • B7h.5 antibody
      • B7h4 antibody
      • B7S1 antibody
      • B7x antibody
      • BC032925 antibody
      • Immune costimulatory protein B7-H4 antibody
      • Immune costimulatory protein B7H4 antibody
      • MGC41287 antibody
      • PRO1291 antibody
      • Protein B7S1 antibody
      • RP11 229A19.4 antibody
      • T cell costimulatory molecule B7x antibody
      • T-cell costimulatory molecule B7x antibody
      • V set domain-containing T cell activation inhibitor 1 antibody
      • V-set domain-containing T-cell activation inhibitor 1 antibody
      • VCTN1 antibody
      • Vtcn1 antibody
      • VTCN1_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Immunohistochemical analysis of paraffin-embedded mouse breast carcinoma tissue labeling B7H4 with ab252438 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Positive staining on mouse breast cancer cells. The section was incubated with ab252438 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Immunohistochemical analysis of paraffin-embedded mouse breast tissue labeling B7H4 with ab252438 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Positive staining on mouse breast. The section was incubated with ab252438 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Immunoprecipitation - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunoprecipitation - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      B7H4 was immunoprecipitated from 0.35 mg SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab252438 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252438 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: SK-BR-3 whole cell lysate 10 ug

      Lane 2: ab252438 IP in SK-BR-3 whole cell lysate 10 ug

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252438 in SK-BR-3 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 127 seconds

      The bands at 65kDa represent glycosylated B7H4 (PMID: 15878339).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Flow Cytometry - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Flow Cytometry - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Flow cytometric analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell) cells labelling B7H4 with ab252438 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T+OE-612 cells labelling B7H4 with ab252438 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cell line transfected with HA-tagged B7H4 expression vector. ab236632 Anti-HA tag rabbit monoclonal antibody was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling B7H4 with ab252438 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast carcinoma (PMID: 15756008). The section was incubated with ab252438 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling B7H4 with ab252438 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human ovarian carcinoma (PMID: 27439899). The section was incubated with ab252438 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling B7H4 with ab252438 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast (PMID: 27439899). The section was incubated with ab252438 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252438).

    • Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)
      Anti-B7H4 antibody [EPR23665-20] - BSA and Azide free (ab272707)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272707 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272707 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab272707.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com