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リコンビナントRabMAb

Anti-B7H4 抗体 [EPR20236] (ab209242)

Submit a review Submit a question References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)
  • Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236] (ab209242)
  • Western blot - Anti-B7H4 antibody [EPR20236] (ab209242)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)
  • Anti-B7H4 antibody [EPR20236] (ab209242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20236] to B7H4
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-B7H4 antibody [EPR20236]
    B7H4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20236] to B7H4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: SK-BR-3 and T-47D whole cell lysates; Human breast cancer lysate. IHC-P: Human breast, breast cancer and kidney tissues. ICC/IF: SK-BR-3 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab209242の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/250.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 65, 45, 28, 18 kDa (predicted molecular weight: 31 kDa).
特記事項
ICC/IF
1/250.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 65, 45, 28, 18 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

  • 機能

    Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

  • 組織特異性

    Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.

  • 配列類似性

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Cell membrane. Expressed at the cell surface. A soluble form has also been detected.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B7 family member, H4 antibody
      • B7 H4 antibody
      • B7 homolog 4 antibody
      • B7 superfamily member 1 antibody
      • B7 superfamily, member 1 antibody
      • B7-H4 antibody
      • B7h.5 antibody
      • B7h4 antibody
      • B7S1 antibody
      • B7x antibody
      • BC032925 antibody
      • Immune costimulatory protein B7-H4 antibody
      • Immune costimulatory protein B7H4 antibody
      • MGC41287 antibody
      • PRO1291 antibody
      • Protein B7S1 antibody
      • RP11 229A19.4 antibody
      • T cell costimulatory molecule B7x antibody
      • T-cell costimulatory molecule B7x antibody
      • V set domain-containing T cell activation inhibitor 1 antibody
      • V-set domain-containing T-cell activation inhibitor 1 antibody
      • VCTN1 antibody
      • Vtcn1 antibody
      • VTCN1_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling B7H4 with ab209242 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and weak cytoplasmic staining on tumor cells of human breast cancer is observed [PMID:15756008]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236] (ab209242)
      Immunocytochemistry/ Immunofluorescence - Anti-B7H4 antibody [EPR20236] (ab209242)

      Immunofluorescent analysis of 100% methanol-fixed SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling B7H4 with ab209242 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on SK-BR-3 cell line. 

      The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Western blot - Anti-B7H4 antibody [EPR20236] (ab209242)
      Western blot - Anti-B7H4 antibody [EPR20236] (ab209242)
      All lanes : Anti-B7H4 antibody [EPR20236] (ab209242) at 1/1000 dilution

      Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
      Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
      Lane 3 : Human breast cancer lysate
      Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 18,28,45,65 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      B7H4 is N-glycosylated protein. The level of B7H4 glycosylation varies between different cells and tissues. The bands at 65kDa and 45kDa represent glycosylated B7H4. The bands at 28kDa and 18kDa represent de-glycosylated B7H4. HT-29 and HeLa cell lines are negative controls. (PMID: 15878339).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)

      Immunohistochemical analysis of paraffin-embedded human breast tissue labeling B7H4 with ab209242 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Predominantly apical, luminal membranous staining on human breast epithelium is observed [PMID: 15756008]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (ab209242)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling B7H4 with ab209242 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on distal convoluted renal tubules of human kidney is observed [PMID: 16798883]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-B7H4 antibody [EPR20236] (ab209242)
      Anti-B7H4 antibody [EPR20236] (ab209242)

    参考文献 (8)

    ab209242 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab209242 は 8 報の論文で使用されています。

    • Qin X  et al. Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis. Reprod Biol Endocrinol 19:114 (2021). PubMed: 34289871
    • Hu X  et al. The anti-B7-H4 checkpoint synergizes trastuzumab treatment to promote phagocytosis and eradicate breast cancer. Neoplasia 22:539-553 (2020). PubMed: 32966956
    • Karvas RM  et al. Use of a human embryonic stem cell model to discover GABRP, WFDC2, VTCN1 and ACTC1 as markers of early first trimester human trophoblast. Mol Hum Reprod 26:425-440 (2020). PubMed: 32359161
    • Wang JB  et al. An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer. Nat Commun 11:6352 (2020). PubMed: 33311518
    • Cao H  et al. Clinical value of detecting IQGAP3, B7-H4 and cyclooxygenase-2 in the diagnosis and prognostic evaluation of colorectal cancer. Cancer Cell Int 19:163 (2019). PubMed: 31223291
    • Yuan X  et al. Blockade of Immune-Checkpoint B7-H4 and Lysine Demethylase 5B in Esophageal Squamous Cell Carcinoma Confers Protective Immunity against P. gingivalis Infection. Cancer Immunol Res 7:1440-1456 (2019). PubMed: 31350278
    • Zhou D  et al. Silencing of B7-H4 suppresses the tumorigenicity of the MGC-803 human gastric cancer cell line and promotes cell apoptosis via the mitochondrial signaling pathway. Int J Oncol 52:1267-1276 (2018). PubMed: 29436630
    • Kang FB  et al. B7-H4 overexpression is essential for early hepatocellular carcinoma progression and recurrence. Oncotarget 8:80878-80888 (2017). IHC-P ; Human . PubMed: 29113351

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